Online Purchasing Account You are logged on as Guest. LoginRegister a New AccountShopping cart (Empty)
United States 

Proteinase-activated receptor-4 (extracellular) polyclonal antibody

 
BML-SA652-0050 50 µl 341.00 USD
 
BML-SA652-0200 200 µl 514.00 USD
Do you need bulk/larger quantities?
 

Product Specification

Alternative Name:PAR-4
 
Host:Rabbit
 
Immunogen:Synthetic peptide corresponding to aa 136-150 (with a C149S replacement) of human PAR-4 (proteinase-activated receptor-4).
 
UniProt ID:Q96RI0
 
Species reactivity:Human, Rat
 
Applications:Flow Cytometry, ICC, IHC, WB
 
Positive Control:Included
 
Formulation:Lyophilized from PBS, pH 7.4, containing 1% BSA and 0.025% sodium azide.
 
Reconstitution:Antibody: Reconstitute with 50 µl of deionized water for BML-SA652-0050. Reconstitute with 200 µl deionized water for BML-SA652-0200. This will yield a 0.8 mg/ml concentration.
Control peptide: Reconstitute with 100 µl of deionized water. This will yield a 0.4 mg/ml concentration.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Scientific Background:PAR-4 belongs to a four member family of G protein-coupled receptors (PAR-1 to -4) that are activated as a result of proteolytic cleavage by certain serine proteases, hence their name. In this modality of activation, a specific proteinase cleaves the PAR receptor within a defined sequence in its extracellular N-terminal domain. This cleavage results in the creation of a new N-terminal sequence (tethered ligand), which subsequently binds to a site in the second extracellular loop of the same receptor. This binding results in the coupling of the receptor to G proteins and in the activation of several signal transduction pathways. Different PARs are activated by different proteinases. PAR-4 is activated by both thrombin and trypsin whereas PAR-1 and PAR-3 are activated by thrombin and PAR-2 is activated by trypsin. PAR-4 can be also cleaved and activated by other proteinases such as cathepsin G. The intracellular signaling mechanisms mediated by PAR-4 activation are not completely elucidated but they may involve calcium mobilization downstream of phospholipase C through the Gαq pathway. Tissue distribution of PAR-4 is very broad with the highest expression levels found in lung, testis, pancreas and small intestine. In addition, PAR-4 expression was observed in platelets, megakaryocytes and leukocytes. Studies with platelets derived form PAR-4 knockout mice have established an essential role for PAR-4 in thrombin-induced platelet activation.PAR-4 is likely involved in other physiological functions such as regulation of gastrointestinal motility and regulation of vascular endothelial cell function.
 

Product Toolbox

PRODUCT RESOURCES

Datasheet
MSDS
Certificate of Analysis

RELATED PRODUCTS

By target:
Proteinase-activated receptor
By biological activity:
Proteinase-activated receptor Polyclonal antibody
By catalog section:

PRODUCT SUPPORT

FAQs
Technical Service
Customer Service

Recommend this page

 
For Research Use Only. Not for use in diagnostic procedures.
Keep in touch

©2018 Enzo Life Sciences, Inc.,