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Neuropeptide Y2 receptor (rat) polyclonal antibody

 
BML-SA643-0200 200 µl 517.00 USD
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Product Specification

Alternative Name:NPY2R
 
Host:Rabbit
 
Immunogen:Synthetic peptide corresponding to aa 346-364 of mouse NPY2R (neuropeptide Y2 receptor).
 
UniProt ID:P97295
 
Species reactivity:Rat
 
Applications:ICC, WB
 
Blocking Peptide:Included: Neuropeptide Y2 Receptor Control Peptide (40µg). Supplied lyophilized.
 
Purity Detail:Affinity purified IgG.
 
Formulation:Lyophilized from PBS, pH 7.4, 1% BSA and 0.05% sodium azide.
 
Reconstitution:To lyophilized antibody, add 50µl or 200µl (depending on which size was purchased) of deionized water. To lyophilized peptide, add 100µl of deionized water.
 
Use/Stability:Lyophilized antibody and lyophilized peptide can be stored intact at room temperature for several weeks. For longer periods they should be stored at -20°C. After reconstitution, the antibody solution can be stored at 4°C for up to 2 weeks. For longer periods, small aliquots should be stored at -20°C or below.Centrifuge all antibody preparations before use (10,000 X g for 5 minutes). After reconstitution, control peptide should be stored at -20°C.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Scientific Background:The NPY receptors belong to the G protein-coupled receptor superfamily. Activation of these receptors by NPY affects [Ca2+]i, adenylate cyclase, and a number of ion channels. NPY2R is primarily expressed in neurons as a presynaptic receptor. It is also strongly expressed in epithelia of the visceral tissues, including colon and kidney. NPY2R is involved in inhibition of neurotransmitter release mediated by NPY. Release of glutamate, the principal neuroexcitatory transmitter, is inhibited via the NPY2R receptor.
 
Technical Info/Product Notes:PREADSORPTION CONTROL: 1µg peptide per 1µg antibody. Prepare 2 tubes. In the first, dilute the necessary amount of antibody in 200-500µl of PBS, 1% BSA and 0.025% sodium azide. Prepare the second tube identically to the first with the addition of the appropriate amount of peptide. Incubate at room temperature for 1 hr. Centrifuge both tubes at 10,000Xg for 5 min. Use the supernatants from tubes 1 and 2 for parallel experiments.
 
BML-SA643 WB
Western blot analysis of rat hippocampus and rat whole brain lysates: Lanes 1, 2. Anti-NPY2R (1:200). Lanes 3, 4. Anti-NPY2R antibody, preincubated with the control peptide
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BML-SA643 WB

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