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Neuropeptide Y1 receptor polyclonal antibody

 
BML-SA642-0050 50 µl 316.00 USD
 
BML-SA642-0200 200 µl 539.00 USD
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Product Specification

Alternative Name:NPY1R
 
Host:Rabbit
 
Immunogen:Synthetic peptide corresponding to aa 237-252 of human NPY1R (neuropeptide Y1 receptor).
 
UniProt ID:P25929
 
Species reactivity:Human, Rat
 
Applications:ICC, IHC, WB
 
Blocking Peptide:Included: Neuropeptide Y1 Receptor Control Peptide (40µg). Supplied lyophilized.
 
Purity Detail:Affinity purified IgG.
 
Formulation:Lyophilized from PBS, pH 7.4, 1% BSA and 0.05% sodium azide.
 
Reconstitution:To lyophilized antibody, add 50µl or 200µl (depending on which size was purchased) of deionized water. To lyophilized peptide, add 100µl of deionized water.
 
Use/Stability:Lyophilized antibody and lyophilized peptide can be stored intact at room temperature for several weeks. For longer periods they should be stored at -20°C. After reconstitution, the antibody solution can be stored at 4°C for up to 2 weeks. For longer periods, small aliquots should be stored at -20°C or below.Centrifuge all antibody preparations before use (10,000 X g for 5 minutes). After reconstitution, control peptide should be stored at -20°C.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Scientific Background:The NPY receptors belong to the G protein-coupled receptor superfamily. Activation of these receptors by NPY affects [Ca2+]i, adenylate cyclase, and a number of ion channels. NPY1R is considered to be a postsynaptic receptor. In the CNS, NPY1Rs are concentrated in the cortical areas of the brain, cerebral cortex, olfactory nucleus, hippocampus, hypothalamic nuclei, and thalamic nuclei. In addition, this receptor has a bimodal role in the immune system, serving as a strong negative regulator on T cells, as well as an activator of antigen-presenting cell function.
 
Technical Info/Product Notes:PREADSORPTION CONTROL: 1µg peptide per 1µg antibody. Prepare 2 tubes. In the first, dilute the necessary amount of antibody in 200-500µl of PBS, 1% BSA and 0.025% sodium azide. Prepare the second tube identically to the first with the addition of the appropriate amount of peptide. Incubate at room temperature for 1 hr. Centrifuge both tubes at 10,000Xg for 5 min. Use the supernatants from tubes 1 and 2 for parallel experiments.
 
BML-SA642 WB
Western blot analysis of Jurkat cells (lanes 1, 4); K562 cells (lanes 2, 5); RBL cells (lanes 3, 6); rat brain lysates (lanes 7, 8). Lanes 1, 2, 3, 7: Anti-NPY1R. Lanes 4, 5, 6, 8: Anti-NPY1R preincubated with the control peptide.
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BML-SA642 WB

Product Literature References

Neuropeptide Y receptor 1 is expressed by B and T lymphocytes and mast cells in infantile haemangiomas: E.M. Tan, et al.; Acta Paediatr. 106, 292 (2017), Abstract;

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