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mGluR1 (extracellular) polyclonal antibody

BML-SA610-0050 50 µl 345.00 USD
BML-SA610-0200 200 µl 517.00 USD
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Product Specification

Alternative Name:Metabotropic glutamate receptor 1, GRM1
Immunogen:Synthetic peptide corresponding to aa 501-516 of rat mGluR1 (GRM1).
UniProt ID:P23385
Species reactivity:Human, Mouse, Rat
Applications:Flow Cytometry, ICC, IP, WB
Formulation:Lyophilized from PBS, pH 7.4, 1% BSA and 0.025% sodium azide.
Reconstitution:Reconstitute in 50 µl or 200 µl deionized water, depending on the sample size.
Shipping:Shipped on Blue Ice
Long Term Storage:-20°C
Scientific Background:mGluR1 and mGluR5 receptors signal through Gq/G11 that activates phospholipase C and ultimately produces an increase in inositol triphosphate and cytosolic Ca2+. More downstream signaling pathways include activation of PKC and modulation of Ca2+ and K+ ion channels. Activation of signaling pathways independent of G proteins has also been reported. mGluR1 is predominantly expressed in nervous tissue although expression in several non-neural cell types has also been described. In the brain it is highly expressed in the hippocampus, cerebellum, olfactory bulb and thalamus.
BML-SA610 Flow
Indirect flow cytometry analysis in live intact Jurkat cells:
Black: Unstained cells + goat-anti-rabbit-FITC.
Green: Cells + Anti-mGluR1 (extracellular) antibody (Cat# BML-SA610), (10 µg) + goat-anti-rabbit-FITC.
Immunoprecipitation of rat brain lysate:
1. Cell lysate + protein A beads + Anti-mGluR1 (extracellular) antibody (Prod. No. BML-SA610).
2. Cell lysate + protein A beads + pre-immune rabbit serum.
3. Cell lysate.
Black arrow indicates the mGluR1 protein (glycosylated form) while the red arrow shows the IgG heavy chain. Immunoblot was performed with the Anti-mGluR1 (extracellular) antibody.
Expression of mGluR1 in rat C6 glioma cells: Immunocytochemical staining of live intact rat C6 glioma cells using Anti-mGluR1 (extracellular) antibody (Cat# BML-SA610), (1:100), followed by goat-anti-rabbit-AlexaFluor-555 secondary antibody (red). Nuclei were stained with Hoechst 33342 (blue).
Western blot analysis of rat brain lysate: 1. Anti-mGluR1 (extracellular) antibody (Cat# BML-SA610), (1:200). 2. Anti-mGluR1 (extracellular) antibody, preincubated with the control peptide antigen.
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Product Literature References

Metabotropic glutamate receptors (mGluR5) activate transient receptor potential canonical channels to improve the regularity of the respiratory rhythm generated by the pre-Bötzinger complex in mice: F. Ben-Mabrouk, et al.; Eur. J. Neurosci. 35, 1725 (2012), Abstract;
Deleterious Effects of Amyloid β Oligomers Acting as an Extracellular Scaffold for mGluR5: M. Renner, et al.; Neuron 66, 739 (2010), Abstract;
Disassembly of shank and homer synaptic clusters is driven by soluble beta-amyloid(1-40) through divergent NMDAR-dependent signalling pathways: F. Roselli, et al.; PLoS One 4, e6011 (2009), Abstract;

General Literature References

Metabotropic glutamate receptors: F. Ferraguti, et al.; Cell Tissue Res. 326, 483 (2006), Abstract;
Regulation of metabotropic glutamate receptor signaling, desensitization and endocytosis: G.K. Dhami, et al.; Pharmacol. Ther. 111, 260 (2006), Abstract;
Ionotropic and metabotropic glutamate receptor structure and pharmacology: J.N. Kew, et al.; Psychopharmacology (Berl) 179, 4 (2005), Abstract;

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