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mGluR1 (extracellular) polyclonal antibody

 
BML-SA610-0050 50 µl 363.00 USD
 
BML-SA610-0200 200 µl 543.00 USD
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Product Specification

Alternative Name:Metabotropic glutamate receptor 1, GRM1
 
Host:Rabbit
 
Immunogen:Synthetic peptide corresponding to aa 501-516 of rat mGluR1 (GRM1).
 
UniProt ID:P23385
 
Species reactivity:Human, Mouse, Rat
 
Applications:Flow Cytometry, ICC, IP, WB
 
Formulation:Lyophilized from PBS, pH 7.4, 1% BSA and 0.025% sodium azide.
 
Reconstitution:Reconstitute in 50 µl or 200 µl deionized water, depending on the sample size.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Scientific Background:mGluR1 and mGluR5 receptors signal through Gq/G11 that activates phospholipase C and ultimately produces an increase in inositol triphosphate and cytosolic Ca2+. More downstream signaling pathways include activation of PKC and modulation of Ca2+ and K+ ion channels. Activation of signaling pathways independent of G proteins has also been reported. mGluR1 is predominantly expressed in nervous tissue although expression in several non-neural cell types has also been described. In the brain it is highly expressed in the hippocampus, cerebellum, olfactory bulb and thalamus.
 
mGluR1 (extracellular) polyclonal antibody Flow Cytometry
Indirect flow cytometry analysis in live intact Jurkat cells:
Black: Unstained cells + goat-anti-rabbit-FITC.
Green: Cells + Anti-mGluR1 (extracellular) antibody (Cat# BML-SA610), (10 µg) + goat-anti-rabbit-FITC.
mGluR1 (extracellular) polyclonal antibody Immunoprecipitation
Immunoprecipitation of rat brain lysate:
1. Cell lysate + protein A beads + Anti-mGluR1 (extracellular) antibody (Prod. No. BML-SA610).
2. Cell lysate + protein A beads + pre-immune rabbit serum.
3. Cell lysate.
Black arrow indicates the mGluR1 protein (glycosylated form) while the red arrow shows the IgG heavy chain. Immunoblot was performed with the Anti-mGluR1 (extracellular) antibody.
mGluR1 (extracellular) polyclonal antibody Immunocytochemistry
Expression of mGluR1 in rat C6 glioma cells: Immunocytochemical staining of live intact rat C6 glioma cells using Anti-mGluR1 (extracellular) antibody (Cat# BML-SA610), (1:100), followed by goat-anti-rabbit-AlexaFluor-555 secondary antibody (red). Nuclei were stained with Hoechst 33342 (blue).
mGluR1 (extracellular) polyclonal antibody Western blot
Western blot analysis of rat brain lysate: 1. Anti-mGluR1 (extracellular) antibody (Cat# BML-SA610), (1:200). 2. Anti-mGluR1 (extracellular) antibody, preincubated with the control peptide antigen.
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mGluR1 (extracellular) polyclonal antibody Flow Cytometry mGluR1 (extracellular) polyclonal antibody Immunoprecipitation mGluR1 (extracellular) polyclonal antibody Immunocytochemistry mGluR1 (extracellular) polyclonal antibody Western blot

Product Literature References

Metabotropic glutamate receptors (mGluR5) activate transient receptor potential canonical channels to improve the regularity of the respiratory rhythm generated by the pre-Bötzinger complex in mice: F. Ben-Mabrouk, et al.; Eur. J. Neurosci. 35, 1725 (2012), Abstract;
Deleterious Effects of Amyloid β Oligomers Acting as an Extracellular Scaffold for mGluR5: M. Renner, et al.; Neuron 66, 739 (2010), Abstract;
Disassembly of shank and homer synaptic clusters is driven by soluble beta-amyloid(1-40) through divergent NMDAR-dependent signalling pathways: F. Roselli, et al.; PLoS One 4, e6011 (2009), Abstract;

General Literature References

Metabotropic glutamate receptors: F. Ferraguti, et al.; Cell Tissue Res. 326, 483 (2006), Abstract;
Regulation of metabotropic glutamate receptor signaling, desensitization and endocytosis: G.K. Dhami, et al.; Pharmacol. Ther. 111, 260 (2006), Abstract;
Ionotropic and metabotropic glutamate receptor structure and pharmacology: J.N. Kew, et al.; Psychopharmacology (Berl) 179, 4 (2005), Abstract;

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