P2Y11 receptor, pAb

[P2RY11]



BML-SA587-0050	50 µl	192.00 USD

BML-SA587-0200	200 µl	422.00 USD

Product Specification

Concentration:	0.8mg/ml after reconstitution.

Purity Detail:	Affinity purified.

Formulation:	Lyophilized from PBS, pH 7.4, containing 1% BSA and 0.05% sodium azide.

Immunogen:	Synthetic peptide corresponding to aa 357-373 of human P2Y11.

Source/Host:	From rabbit.

Reconstitution:	Reconstitute with 50μl or 200μl deionized water. Further dilutions should be made with medium containing a carrier protein such as 1% BSA.

Specificity:	Recognizes human and rat P2Y11.

Application:	Immunohistochemistry Western blot (1:200)

Shipping:	SHIPPED ON BLUE ICE

Long Term Storage:	-20°C

Use/Stability:	The antibody solution can be stored at +4°C for up to 2 weeks.

Handling:	After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.

Miscellaneous/General:	The P2Y receptors belong to the G-protein coupled receptors superfamily. P2Y receptors mediate the actions of the extracellular nucleotides (ATP, ADP, UTP and UDP) and regulate several physiologic responses, among them, cardiac function, platelet aggregation and SMC proliferation. The human P2Y11 receptor is unique among the P2Y receptors since it is coupled to both phospholipase C and adenylyl cyclase. P2Y11 receptor is highly expressed in spleen and to a much lower extent in platelets.

Background / Technical Information:	Preadsorption control: Use 1µg control peptide per 1µg antibody. Prepare 2 tubes. In the first, dilute the necessary amount of antibody in 200-500µl of PBS, 1% BSA and 0.025% sodium azide. Prepare the second tube identically to the first with the addition of the appropriate amount of peptide. Incubate at room temperature for 1 hr. Centrifuge both tubes at 10,000Xg for 5 min. Use the supernatants from tubes 1 and 2 for parallel experiments.