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Endothelin receptor A (rat) polyclonal antibody

 
BML-SA576-0050 50 µl 345.00 USD
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Product Specification

Alternative Name:ET-A
 
Host:Rabbit
 
Immunogen:Synthetic peptide corresponding to aa 413-426 of rat ET-A.
 
UniProt ID:P26684
 
Species reactivity:Rat
 
Applications:IHC, WB
 
Blocking Peptide:Included: ET-A Control Peptide (40µg). Supplied lyophilized.
 
Purity Detail:Affinity purified IgG.
 
Formulation:Lyophilized from PBS, pH 7.4, 1% BSA and 0.05% sodium azide.
 
Reconstitution:To lyophilized antibody, add 50µl or 200µl (depending on which size was purchased) of deionized water. Further dilutions should be made using a carrier protein such as 1% BSA. To lyophilized control peptide, add 100µl of distilled water.
 
Use/Stability:Lyophilized antibody and lyophilized peptide can be stored intact at room temperature for several weeks. For longer periods they should be stored at -20°C. After reconstitution, the antibody solution can be stored at 4°C for up to 2 weeks. For longer periods, small aliquots should be stored at -20°C or below. Centrifuge all antibody preparations before use (10,000 X g for 5 minutes). After reconstitution, control peptide should be stored at -20°C.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Technical Info/Product Notes:PREADSORPTION CONTROL: 1µg peptide per 1µg antibody. Prepare 2 tubes. In the first, dilute the necessary amount of antibody in 200-500µl of PBS, 1% BSA and 0.05% sodium azide. Prepare the second tube identically to the first with the addition of the appropriate amount of peptide. Incubate at room temperature for 1 hr. Centrifuge both tubes at 10,000Xg for 5 min. Use the supernatants from tubes 1 and 2 for parallel experiments.
 

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