14-3-3β, pAb



BML-SA474-0025	25 µl	95.00 USD

BML-SA474-0100	100 µl	328.00 USD

Replaces Prod. #: ALX-215-021

Product Specification

Alternative Name:	KCIP-1, Protein kinase C inhibitor protein 1, Protein 1054, 14-3-3α

Formulation:	Liquid. Neat serum.

Immunogen:	Acetylated N-terminal domain of 14-3-3 β.

Source/Host:	From rabbit.

Specificity:	Recognizes human, mouse, rat, bovine, sheep 14-3-3β. Is highly specific for the β isoform.

Application:	Affinity Chromatography ELISA Immunohistochemistry (1:600) Western Blot (HRP/ECL detection (1:3'000)) Optimal conditions must be determined individually for each application.

Long Term Storage:	-20°C

Background / Technical Information:	Please click here for the comprehensive product datasheet.

Figure: Western blot analysis of cell lysates using 14-3-3 β, pAb (Prod. No. BML-SA474). Antibody dilution 1:3’000, blot was developed by HRP/ECL.1. HeLa S100 (cytosol)2. HeLa (whole cell)3. Jurkat 4. MDCK 5. A4316. MCF-77. rat brain 8. PC12

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Product Literature References

Unchanged survival rates of 14-3-3gamma knockout mice after inoculation with pathological prion protein: P. Steinacker, et al.; Mol. Cell. Biol. 25, 1339 (2005), Abstract; Full Text

Interaction of Akt-phosphorylated ataxin-1 with 14-3-3 mediates neurodegeneration in spinocerebellar ataxia type 1: H.K. Chen, et al.; Cell 113, 457 (2003), Abstract;

Functional specificity in 14-3-3 isoform interactions through dimer formation and phosphorylation. Chromosome location of mammalian isoforms and variants: A. Aitken; Plant Mol. Biol. 50, 993 (2002), Abstract;

Immunolocalisation of 14-3-3 isoforms in normal and scrapie-infected murine brain: H.C. Baxter, et al.; Neuroscience 109, 5 (2002), Abstract;

Isoform pattern of 14-3-3 proteins in the cerebrospinal fluid of patients with Creutzfeldt-Jakob disease: J. Wiltfang, et al.; J. Neurochem. 73, 2485 (1999), Abstract;

14-3-3 inhibits the Dictyostelium myosin II heavy-chain-specific protein kinase C activity by a direct interaction: identification of the 14-3-3 binding domain: M. Matto-Yelin, et al.; Mol. Biol. Cell 8, 1889 (1997), Abstract; Full Text

Identification of 14-3-3 proteins in human platelets: effects of synthetic peptides on protein kinase C activation: C.P. Wheeler-Jones, et al.; Biochem. J. 315, 41 (1996), Abstract; Full Text

Expression and structural analysis of 14-3-3 proteins: D.H. Jones, et al.; J. Mol. Biol. 245, 375 (1995), Abstract;

Isoforms of 14-3-3 protein can form homo- and heterodimers in vivo and in vitro: implications for function as adapter proteins: D.H. Jones, et al.; FEBS Lett. 368, 55 (1995), Abstract; Full Text

Purification of 14-3-3 protein and analysis of isoforms in chicken brain: Y. Patel, et al.; Biochim. Biophys. Acta 1222, 405 (1994), Abstract;

Subcellular localisation of 14-3-3 isoforms in rat brain using specific antibodies: H. Martin, et al.; J. Neurochem. 63, 2259 (1994), Abstract;

Antibodies against the major brain isoforms of 14-3-3 protein. An antibody specific for the N-acetylated amino-terminus of a protein: H. Martin, et al.; FEBS Lett. 331, 296 (1993), Abstract; Full Text