Product Details
| Alternative Name: | Sirtuin-5 |
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| Host: | Rabbit |
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| Immunogen: | Recombinant human SIRT5. |
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| UniProt ID: | Q9NXA8 |
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| Source: | Purified from rabbit serum. |
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| Species reactivity: | Human, Mouse, Rat
Bovine
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| Applications: | WB
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| Recommended Dilutions/Conditions: | Western Blot: (1:2,500, Colorimetric, using secondary antibody coupled to alkaline phosphatase and BCIP/NBT as substrate. / 1:3,000, ECL, using secondary antibody coupled HRP)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application. |
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| Application Notes: | Detects a band of ~29 kDa by Western blot. |
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| Purity Detail: | Partially purified from rabbit serum with immunodepletion. |
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| Formulation: | Liquid. In PBS. |
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| Handling: | Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -80°C. |
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| Shipping: | Dry Ice |
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| Long Term Storage: | -80°C |
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| Scientific Background: | SIRT5 is the only human class III sirtuin and as such is the closest homolog to bacterial (e.g. E. coli CobB) and archaeal sirtuins. SIRT5 is an imported mitochondrial protein with a calculated precursor molecular weight of 34kDa. In various human, rat, mouse and bovine cells and tissues, the major reactive band migrates with an apparent molecular weight of ~29kDa. This presumably reflects removal of an N-terminal transit sequence due to mitochondrial import and processing. No cross-reactivity observed with recombinant human sirtuins SIRT1, SIRT2 or SIRT3. |
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| Regulatory Status: | RUO - Research Use Only |
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Western blot analysis of SIRT5, pAb (Prod. No. BML-SA464): Lane 1: MW marker, Lane 2: SIRT5 (human), (recombinant) (His-tag) (Prod. No. BML-SE555), Lane 3: SIRT3 (human), (recombinant) (His-tag) (Prod. No. BML-SE270), Lane 4: SIRT2 (human), (recombinant) (His-tag) (Prod. No. BML-SE251), Lane 5: SIRT1 (human), (recombinant) (His-tag) (Prod. No. BML-SE239).
Western blot analysis of SIRT5, pAb (Prod. No. BML-SA464): Lane 1: MW marker, Lane 2: SIRT5 (human), (recombinant) (His-tag) (Prod. No. BML-SE555), Lane 3: SIRT3 (human), (recombinant) (His-tag) (Prod. No. BML-SE270), Lane 4: SIRT2 (human), (recombinant) (His-tag) (Prod. No. BML-SE251), Lane 5: SIRT1 (human), (recombinant) (His-tag) (Prod. No. BML-SE239).
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General Literature References
Evolutionarily conserved and nonconserved cellular localizations and functions of human SIRT proteins: E. Michishita, et al.; Mol. Biol. Cell
16, 4623 (2005),
Abstract;
