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SIRT5 polyclonal antibody

 
BML-SA464-0100 100 µl 313.00 USD
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Product Specification

Alternative Name:Sirtuin-5
 
Host:Rabbit
 
Immunogen:Recombinant human SIRT5.
 
UniProt ID:Q9NXA8
 
Species reactivity:Human, Mouse, Rat
Bovine
 
Applications:WB
 
Recommended Dilutions/Conditions:Western Blot: (1:2,500, Colorimetric, using secondary antibody coupled to alkaline phosphatase and BCIP/NBT as substrate. / 1:3,000, ECL, using secondary antibody coupled HRP)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Application Notes:Detects a band of ~29 kDa by Western blot.
 
Purity Detail:Partially purified from rabbit serum with immunodepletion.
 
Formulation:Liquid. In PBS.
 
Handling:Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -80°C.
 
Shipping:Shipped on Dry Ice
 
Long Term Storage:-80°C
 
Scientific Background:SIRT5 is the only human class III sirtuin and as such is the closest homolog to bacterial (e.g. E. coli CobB) and archaeal sirtuins. SIRT5 is an imported mitochondrial protein with a calculated precursor molecular weight of 34kDa. In various human, rat, mouse and bovine cells and tissues, the major reactive band migrates with an apparent molecular weight of ~29kDa. This presumably reflects removal of an N-terminal transit sequence due to mitochondrial import and processing. No cross-reactivity observed with recombinant human sirtuins SIRT1, SIRT2 or SIRT3.
 
BML-SA464 WB Colorimetric
Western blot analysis of SIRT5, pAb (Prod. No. BML-SA464): Lane 1: MW marker, Lane 2: SIRT5 (human), (recombinant) (His-tag) (Prod. No. BML-SE555), Lane 3: SIRT3 (human), (recombinant) (His-tag) (Prod. No. BML-SE270), Lane 4: SIRT2 (human), (recombinant) (His-tag) (Prod. No. BML-SE251), Lane 5: SIRT1 (human), (recombinant) (His-tag) (Prod. No. BML-SE239).
BML-SA464 WB ECL
Western blot analysis of SIRT5, pAb (Prod. No. BML-SA464): Lane 1: MW marker, Lane 2: SIRT5 (human), (recombinant) (His-tag) (Prod. No. BML-SE555), Lane 3: SIRT3 (human), (recombinant) (His-tag) (Prod. No. BML-SE270), Lane 4: SIRT2 (human), (recombinant) (His-tag) (Prod. No. BML-SE251), Lane 5: SIRT1 (human), (recombinant) (His-tag) (Prod. No. BML-SE239).
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BML-SA464 WB Colorimetric BML-SA464 WB ECL

General Literature References

Evolutionarily conserved and nonconserved cellular localizations and functions of human SIRT proteins: E. Michishita, et al.; Mol. Biol. Cell 16, 4623 (2005), Abstract;

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