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SIRT3 polyclonal antibody

 
BML-SA463-0100 100 µl 313.00 USD
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Product Specification

Alternative Name:Sirtuin-3
 
Host:Rabbit
 
Immunogen:Recombinant human SIRT3.
 
UniProt ID:Q9NTG7
 
Species reactivity:Human, Rat
Bovine
 
Applications:IHC (PS), WB
 
Recommended Dilutions/Conditions:Western Blot (1:1,000)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Formulation:Liquid. In PBS.
 
Handling:Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.
 
Shipping:Shipped on Dry Ice
 
Long Term Storage:-20°C
 
Scientific Background:SIRT3 is synthesized as an inactive precursor protein whose mature, active form is located in the mitochondrial matrix and/or on the inner mitochondrial membrane. Research showing that caloric restriction elevates SIRT3 expression in mouse adipose tissue and the absence of a SIRT3 allele lacking an enhancer element in long-lived men suggest a possible connection between SIRT3 expression and longevity.
 
BML-SA463 IHC
Immunohistochemistry staining of formalin fixed, paraffin-embedded human liver tumor tissue using SIRT3 antibody (Prod. no. BML-SA463) showing cell membrane staining of hepatocytes.
BML-SA463 WB1
Western blot analysis of recombinant human sirtuins, cell and tissue extracts. Blots were probed with SIRT3, pAb at 1/1000. Secondary antibodies were coupled to alkaline phosphatase and developed with BCIP/NBT . Arrows indicate positions of precursor (P) and mature (M) forms of SIRT3. Numbers indicate molecular weights of marker proteins in kDa. Lanes: M-MW markers, B-bovine heart, R-rat thymus, S3-recomb. SIRT3, 50 ng, L-HL60 cells, Hn-HeLa nucleus, S5-recomb. SIRT5, 50 ng, J-Jurkat cells, Hc-HeLa cytosol, and S2-recomb. SIRT2, 50 ng.
BML-SA463 WB2
Western blot analysis of recombinant human sirtuins, cell and tissue extracts. Blots were probed with SIRT3, pAb at 1/1000. Secondary antibodies were coupled to horseradish peroxidase and developed by ECL. Arrows indicate positions of precursor (P) and mature (M) forms of SIRT3. Numbers indicate molecular weights of marker proteins in kDa. Lanes: M-MW markers, L-HL60 cells, and J-Jurkat cells.
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BML-SA463 IHC BML-SA463 WB1 BML-SA463 WB2

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