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Caspase-3 (human) polyclonal antibody

 
BML-SA320-0100 100 µl 313.00 USD
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Product Specification

Host:Rabbit
 
Immunogen:Recombinant human caspase-3.
 
UniProt ID:P42574
 
Species reactivity:Human
 
Specificity:Recognizes the proenzyme (32 kDa) and the large (17 kDa) and small (12 kDa) subunits of mature caspase-3.
 
Crossreactivity:Does not cross-react with other caspases (caspases-1, -2, -4, -10, -14).
 
Applications:WB
 
Formulation:Liquid. Neat serum containing 0.09% sodium azide.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Protocol:Western Blotting using Anti-Caspase Antibodies (BML-SA320, BML-SA321) and HL60 Cell Extracts (BML-SW101, BML-SW102)
  1. HL60 cell extracts were prepared from exponentially growing cells either induced for apoptosis with etoposide
 
BML-SA320 WB1
Western Blot Analysis of Caspase 3 (human), pAb (BML-SA320): Lane 1: MW Marker, Lane 2: Jurkat Cell Lysate, Lane 3: Jurkat + Staurosporine Cell Lysate, Lane 4: MCF-7 Cell Lysate
BML-SA320 WB2
Western blot analysis of whole cell extracts from uninduced Jurkat cells (-) and Jurkat cells induced to BS19undergo apoptosis (+) by treatment with human, recombinant soluble TRAIL [SE-721]. One hundred thousand cells were used per lane. The nitrocellulose membrane was probed with Anti-Caspase-3 [SA-320] at 1/5000. Secondary antibody was GAR-AP (1/2000) and the nitrocellulose was developed with BCIP/NBT. P and L indicate positions of the proenzyme and large subunit of the active enzyme, respectively.
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BML-SA320 WB1 BML-SA320 WB2

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