PAb421 antibody induces allosteric changes in certain tumor-derived p53 mutants and restores tumor suppressor activity. Microinjection of PAb421 into SW480 colorectal carcinoma cells restored transcriptional activation function to the resident mutant p53 (Arg to His 273, Pro to Ser 309). The epitope for PAb421 is contained within the "p53 activator peptide" 46. Thus the antibody recognizes this peptide and has been used to examine cellular uptake of the p53 activator peptide.
Product Details
| Alternative Name: | TRP53, Tumor protein p53 |
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| Clone: | PAb421 |
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| Host: | Mouse |
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| Isotype: | IgG2a |
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| Immunogen: | Synthetic peptide corresponding to aa 371-380 of human p53. |
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| UniProt ID: | P04637 |
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| Species reactivity: | Human, Mouse, Rat
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| Applications: | ICC, IHC, IP, WB
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| Recommended Dilutions/Conditions: | Western Blot (10µg/ml)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application. |
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| Purity Detail: | Purified. |
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| Formulation: | Liquid. In 50 mM sodium phosphate buffer, pH 7.5, containing 0.2% gelatin and 0.09% sodium azide. |
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| Handling: | Avoid freeze/thaw cycles. |
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| Shipping: | Blue Ice |
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| Long Term Storage: | +4°C |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Heteroclitic serological response in esophageal and prostate cancer patients after NY-ESO-1 protein vaccination: J. Kawada, et al.; Int. J. Cancer
130, 584 (2012),
Application(s): WB,
Abstract;
Full Text
miR-605 joins p53 network to form a p53:miR-605:Mdm2 positive feedback loop in response to stress: J. Xiao, et al.; EMBO J.
30, 5021 (2011),
Abstract;
Transcriptional activation of the tumor suppressor and differentiation gene S100A2 by a novel p63-binding site: R.D. Kirschner, et al.; Nucleic Acids Res.
36, 2969 (2008),
Application(s): Used for electrophoretic mobility shift assay (EMSA),
Abstract;
Full Text
General Literature References
Doxorubicin and octreotide induce a 40 kDa breakdown product of p53 in human hepatoma and tumoral colon cell lines: Z. Sadji-Ouatas et al.; Biochem. J.
364, 881 (2002),
Abstract;
Restoration of the growth suppression function of mutant p53 by a synthetic peptide derived from the p53 C-terminal domain: G. Selivanova et al.; Nat, Med,
3, 632 (1997),
Abstract;
Microinjection of monoclonal antibody PAb421 into human SW480 colorectal carcinoma cells restores the transcription activation function to mutant p53: P. Abarzua et al.; Cancer Res.
55, 3490 (1995),
Abstract;
