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[pThr¹⁸⁵/pTyr¹⁸⁷]ERK1/2 polyclonal antibody



BML-SA275-0020	20 µl	187.00 USD
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Product Details

Alternative Name:	MAPK2/3, MAP kinase 2/3

Host:	Rabbit

Immunogen:	Synthetic peptide corresponding to a portion of human ERK1&2 phosphorylated at Thr^202/185 and Tyr^204/187. This region is conserved among many species including mouse, rat, bovine, frog and fly.

UniProt ID:	P27361 (Erk1), P28482 (Erk2)

Species reactivity:	Human, Mouse, Rat Chicken

Specificity:	Recognizes ERK1/2 phosphorylated at pThr¹⁸⁵/Tyr¹⁸⁷.

Applications:	IHC, WB

Purity Detail:	Purified by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the phosphopeptide used as the immunogen. The final product was generated by affinity chromatography.

Formulation:	Liquid. In Dulbecco’s PBS, pH 7.3, containing 50% glycerol, 1.0 mg/ml BSA, and 0.05% sodium azide.

Shipping:	Dry Ice

Long Term Storage:	-20°C

Regulatory Status:	RUO - Research Use Only

[pThr185/pTyr187]ERK1/2 polyclonal antibody Western blot

A. ERK1 & 2 (pTpY 185/187). B. ERK1 & 2.
PC12 cells were treated with NGF and NIH3T3 cells were treated with PDGF and cell extracts were immunoblotted with either [pThr¹⁸⁵/pTyr¹⁸⁷]ERK1/2, pAb (Prod. No. BML-SA275) or ERK1/2, pAb (Prod. No. BML-SA278).

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