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PARP-1 monoclonal antibody (C-2-10)

 
BML-SA250-0050 50 µl 301.00 USD
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Product Specification

Alternative Name:Poly(ADP-ribose) polymerase-1
 
Clone:C-2-10
 
Host:Mouse
 
Isotype:IgG1
 
Immunogen:Purified calf thymus poly(ADP-ribose) polymerase (PARP).
 
UniProt ID:P18493
 
Species reactivity:Human, Mouse, Rat
Bovine, Hamster, Monkey
 
Specificity:Recognizes an epitope in the C-terminal part of the DNA binding domain of PARP.
 
Crossreactivity:Does not cross-react with chicken PARP.
 
Applications:ELISA, IHC, WB
 
Application Notes:Detects bands ~116kDa (intact PARP) and ~85kDa (apoptosis-induced cleavage fragment) by Western blot.
 
Formulation:Liquid. Mouse ascites containing 0.02% sodium azide.
 
Handling:Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
BML-SA250 WB
Western blot analysis of whole cell extracts from (1) human HL60 leukemia cells (Prod. No. BML-SW101] and (2) HL60 cells induced to undergo apoptosis using etoposide (Prod. No. BML-SW102). The membrane was probed with C-2-10 mouse monoclonal antibody (Prod. No. BML-SA250) at 1:1000 dilution, secondary antibody was GAMAP (1:2000) and developed with BCIP/NBT. Arrows correspond to 116kDa and 85kDa.
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BML-SA250 WB

Product Literature References

Novel p53 target genes secreted by the liver are involved in non-cell-autonomous regulation: M. Charni, et al.; Cell Death Differ. 23, 509 (2016), Application(s): Western Blot, Abstract;
FK-3000 isolated from Stephania delavayi Diels. inhibits MDA-MB-231 cell proliferation by decreasing NF-κB phosphorylation and COX-2 expression: X. Hu, et al.; Int. J. Oncol. 46, 2309 (2015), Application(s): Western Blot, Abstract; Full Text

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The PARP Family & ADP Ribosylation
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