Online Purchasing Account You are logged on as Guest. LoginRegister a New AccountShopping cart (Empty)
United States 

PARP-1 monoclonal antibody (C-2-10) (affinity purified)

 
BML-SA249-0050 50 µg 367.00 USD
Do you need bulk/larger quantities?
 
Replaces Prod. #: ALX-804-210

Product Specification

Alternative Name:Poly(ADP-ribose) polymerase-1
 
Clone:C-2-10
 
Host:Mouse
 
Isotype:IgG1
 
Immunogen:Purified calf thymus poly(ADP-ribose) polymerase (PARP).
 
UniProt ID:P18493
 
Species reactivity:Human, Mouse, Rat
Bovine, Hamster, Monkey
 
Specificity:Recognizes an epitope in the C-terminal part of the DNA binding domain of PARP.
 
Crossreactivity:Does not cross-react with chicken PARP.
 
Applications:ELISA, ICC, WB
 
Recommended Dilutions/Conditions:Western Blot (1µg/ml, alkaline phosphatase)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Application Notes:Detects bands ~116kDa (intact PARP) and ~85kDa (apoptosis-induced cleavage fragment) by Western blot.
 
Purity Detail:Affinity purified.
 
Formulation:Liquid. In PBS containing 0.02% sodium azide.
 
Handling:Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.
 
Shipping:Shipped on Dry Ice
 
Long Term Storage:-20°C
 
bml-sa249 wb
Western blot analysis of whole cell extracts from (1) human HL60 leukemia cells (Prod. No. BML-SW101) and (2) HL60 cells induced to undergo apoptosis using etoposide (Prod. No. BML-SW102). The nitrocellulose membrane was probed with purified clone C-2-10 mouse monoclonal anti-PARP (Prod. No. BML-SA249) at 1 µg/ml. Secondary antibody was GAM-AP (1/2000) and the nitrocellulose membrane was developed with BCIP/NBT. Arrows correspond to 116kDa and 85kDa.
Please mouse over
bml-sa249 wb

Product Literature References

Autophagy requires poly(adp-ribosyl)ation-dependent AMPK nuclear export: J.M. Rodríguez-Vargas, et al.; Cell Death Differ. 23, 2007 (2016), Application(s): Western blot, MCF7 GFP-LC3 cells, Abstract;
PRIMA-1(MET) induces death in soft-tissue sarcomas cell independent of p53: T. Grellety, et al.; BMC Cancer 15, 684 (2015), Application(s): Immunoblot analysis, Abstract;
Alzheimer's disease-associated polymorphisms in human OGG1 alter catalytic activity and sensitize cells to DNA damage: K.D. Jacob, et al.; Free Radic. Biol. Med. 63, 115 (2013), Application(s): Western blot, Abstract;
Regulation of poly(ADP-ribose) polymerase-1 functions by leukocyte elastase inhibitor/LEI-derived DNase II during caspase-independent apoptosis: C. Leprête, et al.; Int. J. Biochem. Cell Biol. 41, 1046 (2009), Abstract;
CCCTC-binding Factor Activates PARP-1 Affecting DNA Methylation Machinery: T. Guastafierro, et al.; J. Biol. Chem. 283, 21873 (2008), Abstract; Full Text
Nuclear poly(ADP-ribose) polymerase-1 rapidly triggers mitochondrial dysfunction: G. Cipriani, et al.; J. Biol. Chem. 280, 17227 (2005), Abstract; Full Text
Inactivation of p21WAF1 sensitizes cells to apoptosis via an increase of both p14ARF and p53 levels and an alteration of the Bax/Bcl-2 ratio: D. Javelaud & F. Besançon; J. Biol. Chem. 277, 37949 (2002), Abstract; Full Text
Characterization of the necrotic cleavage of poly(ADP-ribose) polymerase (PARP-1): implication of lysosomal proteases: S. Gobeil, et al.; Cell Death Differ. 8, 588 (2001), Abstract;
Selective loss of poly(ADP-ribose) and the 85-kDa fragment of poly(ADP- ribose) polymerase in nucleoli during alkylation-induced apoptosis of HeLa cells: R. Alvarez-Gonzalez, et al.; J. Biol. Chem. 274, 32122 (1999), Abstract; Full Text
Activation of a CrmA-insensitive, p35-sensitive pathway in ionizing radiation-induced apoptosis: R. Datta, et al.; J. Biol. Chem. 272, 1965 (1997), Abstract;
Characterization of anti-peptide antibodies directed towards the automodification domain and apoptotic fragment of poly (ADP-ribose) polymerase: P.J. Duriez, et al.; Biochim. Biophys. Acta 1334, 65 (1997), Abstract;
Different cleavage pattern for poly(ADP-ribose) polymerase during necrosis and apoptosis in HL-60 cells: G.M. Shah, et al.; Biochem. Biophys. Res. Commun. 229, 838 (1996), Abstract;
FADD/MORT1 is a common mediator of CD95 (Fas/APO-1) and tumor necrosis factor receptor-induced apoptosis: A.M. Chinnaiyan, et al.; J. Biol. Chem. 271, 4961 (1996), Abstract; Full Text
Studies of the lamin proteinase reveal multiple parallel biochemical pathways during apoptotic execution: Y.A. Lazebnik, et al.; PNAS 92, 9042 (1995), Abstract; Full Text
Cleavage of poly(ADP-ribose) polymerase by a proteinase with properties like ICE: Y.A. Lazebnik, et al.; Nature 371, 346 (1994), Abstract;
Specific proteolytic cleavage of poly(ADP-ribose) polymerase: an early marker of chemotherapy-induced apoptosis: S.H. Kaufmann, et al.; Cancer Res. 53, 3976 (1993), Abstract;
Structural and functional analysis of poly(ADP ribose) polymerase: an immunological study: D. Lamarre, et al.; Biochim. Biophys. Acta 950, 147 (1988), Abstract;

Related Products

PARP-1 monoclonal antibody (C-2-10) 

ELISA, IHC, WB | Print as PDF
 
BML-SA250-0050 50 µl 301.00 USD
Do you need bulk/larger quantities?
 

PARP polyclonal antibody 

ELISA, WB | Print as PDF
 
BML-SA253-0025 25 µl 129.00 USD
 
BML-SA253-0100 100 µl 313.00 USD
Do you need bulk/larger quantities?
 

HL60 Cell Extract (non-induced) 

Print as PDF
 
BML-SW101-0200 200 µl 44.00 USD
Do you need bulk/larger quantities?
 

HL60 Cell Extract (Etoposide-induced) 

Print as PDF
 
BML-SW102-0200 200 µl 44.00 USD
Do you need bulk/larger quantities?
 

Mouse Thymus Extract Standard 

WB | Print as PDF
 
BML-SW110-0200 200 µl 44.00 USD
Do you need bulk/larger quantities?
 

Rat Thymus Extract Standard 

WB | Print as PDF
 
BML-SW112-0200 200 µl 32.00 USD
Do you need bulk/larger quantities?
 

Jurkat Cell Extract (non-induced) 

WB | Print as PDF
 
BML-SW115-0100 100 µl 50.00 USD
Do you need bulk/larger quantities?
 

Jurkat Cell Extract (induced) 

WB | Print as PDF
 
BML-SW116-0100 100 µl 44.00 USD
Do you need bulk/larger quantities?
 

Product Toolbox

PRODUCT RESOURCES

Datasheet
MSDS
Certificate of Analysis

RELATED PRODUCTS

By target:
PARP
By biological activity:
PARP Monoclonal antibody
By catalog section:

PRODUCT SUPPORT

FAQs
Technical Service
Customer Service

Related Literature

Brochures
Monitor programmed cell death from membrane to nucleus
Monitor programmed cell death from membrane to nucleus
Download as PDF

Product Flyers
The PARP Family & ADP Ribosylation
The PARP Family & ADP Ribosylation
Download as PDF

All new literature pieces

Recommend this page

 
For Research Use Only. Not for use in diagnostic procedures.
Keep in touch

©2017 Enzo Life Sciences, Inc.,