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Aryl hydrocarbon receptor polyclonal antibody (affinity purified)

BML-SA210-0025 25 µg 129.00 USD
BML-SA210-0100 100 µg 378.00 USD
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Product Specification

Alternative Name:AhR
Immunogen:Recombinant protein corresponding to aa 1-402 of the N-terminal B-1 AhR (Aryl hydrocarbon receptor).
UniProt ID:P30561
Species reactivity:Mouse
Crossreactivity:Does not cross-react with Arnt.
Applications:IP, WB
Recommended Dilutions/Conditions:Western Blot (1:2,000)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Application Notes:Detects a band of ~95 kDa by Western blot.
Purity Detail:Protein A affinity purified.
Formulation:Liquid. In TBS containing 0.02% sodium azide.
Handling:Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.
Shipping:Shipped on Dry Ice
Long Term Storage:-20°C
Western blot analysis of Aryl hydrocarbon receptor, pAb (Prod. No. BML-SA210): Lane 1: MW marker; Lane 2: Mouse liver extract; Lane 3: Rat liver extract.
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Product Literature References

Aryl hydrocarbon receptor is required for optimal B-cell proliferation: M. Villa, et al.; EMBO J. 36, 116 (2017), Abstract;
Microbial-Derived 1,4-Dihydroxy-2-naphthoic Acid and Related Compounds as Aryl Hydrocarbon Receptor Agonists/Antagonists: Structure-Activity Relationships and Receptor Modeling: Y. Cheng, et al.; Toxicol. Sci. 155, 458 (2017), Abstract;
Ah Receptor Expression in Cardiomyocytes Protects Adult Female Mice from Heart Dysfunction Induced by TCDD Exposure: H. Kurita, et al.; Toxicology 355-356, 9 (2016), Application(s): Determination of cardiac AHR expression, Abstract;
Binding studies using Pichia pastoris expressed human aryl hydrocarbon receptor and aryl hydrocarbon receptor nuclear translocator proteins: Y. Zheng, et al.; Protein Expr. Purif. 122, 72 (2016), Application(s): LI-COR Western analysis, Abstract;
Genistein decreases basal hepatic cytochrome P450 1A1 protein expression and activity in Swiss Webster mice: E.B. Froyen & F.M. Steinberg; Nutr. Res. 36, 430 (2016), Application(s): Chromatin immunoprecipitation assay, Abstract;
Type I interferons and microbial metabolites of tryptophan modulate astrocyte activity and central nervous system inflammation via the aryl hydrocarbon receptor: V. Rothhammer, et al.; Nat. Med. 22, 586 (2016), Abstract; Full Text
In vitro re-expression of the aryl hydrocarbon receptor (Ahr) in cultured Ahr-deficient mouse antral follicles partially restores the phenotype to that of cultured wild-type mouse follicles: A. Ziv-Gal, et al.; Toxicol. In Vitro 29, 329 (2015), Abstract;
Aryl hydrocarbon receptor plays protective roles in ConA-induced hepatic injury by both suppressing IFN-γ expression and inducing IL-22: H. Abe, et al.; Int. Immunol. 26, 129 (2014), Application(s): IP and WB using mouse liver homogenates, Abstract; Full Text
Identification of amino acid residues in the ligand-binding domain of the aryl hydrocarbon receptor causing the species-specific response to omeprazole: possible determinants for binding putative endogenous ligands: K. Shiizaki, et al.; Mol. Pharmacol. 85, 279 (2014), Application(s): WB using cell lysates, Abstract; Full Text
Novel role of hnRNP-A2/B1 in modulating aryl hydrocarbon receptor ligand sensitivity: S.W. Cho, et al.; Arch. Toxicol. 89, 2027 (2014), Abstract;
Aryl hydrocarbon receptor is a target of 17-Allylamino-17-demethoxygeldanamycin and enhances its anticancer activity in lung adenocarcinoma cells: P.H. Chen, et al.; Mol. Pharmacol. 83, 605 (2013), Application(s): WB using human cell lysates, Abstract; Full Text
Aryl hydrocarbon receptor protects lung adenocarcinoma cells against cigarette sidestream smoke particulates-induced oxidative stress: Y.H. Cheng, et al.; Toxicol. Appl. Pharmacol. 259, 293 (2012), Abstract;
Cadmium modulates expression of aryl hydrocarbon receptor-associated genes in rat uterus by interaction with the estrogen receptor: F.M. Kluxen, et al.; Arch. Toxicol. 86, 591 (2012), Abstract;
TGF-β1 signaling plays a dominant role in the crosstalk between TGF-β1 and the aryl hydrocarbon receptor ligand in prostate epithelial cells: A. Staršíchová, et al.; Cell Signal. 24, 1665 (2012), Abstract;
Identification of the aryl hydrocarbon receptor target gene TiPARP as a mediator of suppression of hepatic gluconeogenesis by 2,3,7,8-tetrachlorodibenzo-p-dioxin and of nicotinamide as a corrective agent for this effect: S. Diani-Moore, et al.; J. Biol. Chem. 285, 38801 (2010), Application(s): ChIP using chick embryo hepatocyte (CEH) homogenates, Abstract; Full Text

General Literature References

ER alpha-AHR-ARNT protein-protein interactions mediate estradiol-dependent transrepression of dioxin-inducible gene transcription: T.V. Beischlag & G.H. Perdew; J. Biol. Chem. 280, 21607 (2005), Abstract;
Agonist and chemopreventative ligands induce differential transcriptional cofactor recruitment by aryl hydrocarbon receptor: E.V. Hestermann & M. Brown; Mol. Cell. Biol. 23, 7920 (2003), Abstract;
The aryl hydrocarbon receptor and aryl hydrocarbon receptor nuclear translocator protein show distinct subcellular localizations in Hepa 1c1c7 cells by immunofluorescence microscopy: R.S. Pollenz, et al.; Mol. Pharmacol. 45, 428 (1994), Abstract;

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