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Nitric oxide synthase (inducible) polyclonal antibody

BML-SA200-0025 25 µl 159.00 USD
BML-SA200-0100 100 µl 476.00 USD
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Product Specification

Alternative Name:iNOS, NOS II
Immunogen:Synthetic peptide corresponding to aa 1131-1141 of mouse macrophage NOS.
UniProt ID:P29477
Gene/Protein Identifier:NM_010927 (RefSeq)
Species reactivity:Human, Mouse, Rat
Crossreactivity:Does not cross-react with eNOS or nNOS.
Applications:ICC, IHC, WB
Recommended Dilutions/Conditions:Immunocytochemistry (1:500)
Immunohistochemistry (1:500)
Western Blot (1:2,000)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Application Notes:Detects a band of ~130kDa by Western blot.
Blocking Peptide:For Blocking peptide see Prod. No. BML-SP200.
Formulation:Liquid. Neat serum.
Handling:Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C.
Shipping:Shipped on Dry Ice
Long Term Storage:-80°C
Western blot analysis of lysate from mouse macrophage RAW 264.7 cell line, which was induced with IFNγ and LPS. The nitrocellulose membrane was probed with Anti-iNOS [Prod. no. BML-SA200] at 1/1000 dilution. Blot was developed with “Vectastain ABC Kit” and BCIP/NBT. Arrow points to MW ~130kDa.
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Product Literature References

Vitamin D2 suppresses amyloid-β 25-35 induced microglial activation in BV2 cells by blocking the NF-κB inflammatory signaling pathway: S. Raha, et al.; Life Sci. 161, 37 (2016), Application(s): Western blot; evaluation of the role of VD2 on anti-inflammation, Abstract;
Low-level laser therapy for prevention of noise-induced hearing loss in rats: A. Tamura, et al.; Neurosci. Lett. 595, 81 (2015), Application(s): Immunohistochemistry, Abstract;
Expression of inducible nitric oxide synthase and cyclooxygenase-2 in ovarian cancer: correlation with clinical outcome: M.R. Raspollini, et al.; Gynecol. Oncol. 92, 806 (2004), Abstract;
Altered expression of inducible nitric oxide synthase (iNOS) in the cochlea: X. Shi, et al.; Hear. Res. 177, 43 (2003), Abstract;
Effect of SNI-2011 on amylase secretion from parotid tissue in rats and in neuronal nitric oxide synthase knockout mice: Z. Yuan, et al.; Eur. J. Pharmacol. 464, 197 (2003), Abstract;
Evidence for a functional nitric oxide synthase system in brown adipocyte nucleus: A. Giordano, et al.; FEBS Lett. 514, 135 (2002), Abstract;

General Literature References

Cloned and expressed macrophage nitric oxide synthase contrasts with the brain enzyme: C.J. Lowenstein, et al.; PNAS 89, 6711 (1992), Abstract;
Molecular cloning and functional expression of an inducible nitric oxide synthase from a murine macrophage cell line: C.R. Lyons, et al.; J. Biol. Chem. 267, 6370 (1992), Abstract;
D. Bredt, et al.; Nature 351, 71 (1991),

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