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p62 (human) polyclonal antibody

BML-PW9860-0025 25 µl 108.00 USD
BML-PW9860-0100 100 µl 345.00 USD
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Product Specification

Alternative Name:SQSTM1, Sequestosome I
Immunogen:Synthetic peptide corresponding to aa 387-436 human p62.
UniProt ID:Q13501
Species reactivity:Human
Applications:ELISA, IF, IHC, WB
Recommended Dilutions/Conditions:Immunohistochemistry (1:1,000)
Western Blot (1:1,000)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Purity Detail:Peptide affinity purified.
Formulation:Liquid. In PBS containing 0.09% sodium azide.
Handling:Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.
Shipping:Shipped on Blue Ice
Long Term Storage:-20°C
Scientific Background:P62, also known as Sequestosome I, is a 62kDa, 440 amino acid protein, initially identified as a ligand of the SH2 domain of p56lck, now known to be expressed in many tissues. In addition to TRAF6, PEST and zinc finger motifs, p62 has a C-terminal ubiquitin binding association (UBA) domain with an affinity for multi-ubiquitin chains, and it is considered to serve as a scaffold protein, capable of binding to multiple signalling molecules and uniting receptor-mediated signalling events with ubiquitinylation. Elevated levels of p62 have been reported in breast tumours and in alcoholic liver disease where p62 has been shown to be involved in the formation of Mallory bodies. Several mutations in the p62 UBA domain have been identified and the etiology of Paget’s disease of bone has been linked to one such mutation. Kuusisto and colleagues have demonstrated that p62 is also present in elevated levels in the hallmark inclusions found in various neurodegenerative conditions, including tauopathies (Alzheimer’s disease, Picks disease, and frontotemporal dementia) and synucleinopathies (Parkinson’s disease, dementia with Lewy body disease and multiple system atrophy). In recent years ubiquitin immunostaining has been used to provide adjunct information for neuropathological diagnosis, but it is becoming evident that p62 may be an even more reliable marker of neurodegenerative disease inclusion detection than tau, alpha-synuclein or ubiquitin immunostaining.
Western blot analysis of wild type p62, GST-tagged (human, recombinant) bound to: (1) glutathione-agarose and (3) ubiquitin-agarose (2: agarose control). Bound species were analysed by PAGE followed by blotting on to PVDF and probing with Prod. No. BML-PW9860.
Immunohistochemistry analysis of p62 immunoreactivity is present in neurons of the hippocampus of an Alzheimer patient. Note the intense reaction in the neurofibrillary tangles (dilution 1:1000).
Micrograph courtesy of Professor Fred van Leeuwen, University of Maastricht, The Netherlands.
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Product Literature References

Autophagy controls the pathogenicity of OPA1 mutations in dominant optic atrophy: M.S. Kane, et al.; J. Cell. Mol. Med. (2017), Abstract; Full Text
Defects in MAP1S-mediated autophagy cause reduction in mouse lifespans especially when fibronectin is overexpressed: W. Li, et al.; Aging Cell 15, 370 (2016), Application(s): Immunoblotting, Abstract; Full Text
Fisetin stimulates autophagic degradation of phosphorylated tau via the activation of TFEB and Nrf2 transcription factors: S. Kim, et al.; Sci. Rep. 6, 24933 (2016), Application(s): Cell culture, Abstract;
Protein degradation in a LAMP-2 deficient B-lymphoblastoid cell line from a patient with Danon disease: R. Sanchez-Lanzas, et al.; Biochim. Biophys. Acta 1862, 1423 (2016), Application(s): Western blot, Abstract;
Toll-like receptor 4 knockout alleviates paraquat-induced cardiomyocyte contractile dysfunction through an autophagy-dependent mechanism: S. Wang, et al. ; Toxicol. Lett. 257, 11 (2016), Application(s): Western blot, Abstract;
Tyrosinase-Cre-Mediated Deletion of the Autophagy Gene Atg7 Leads to Accumulation of the RPE65 Variant M450 in the Retinal Pigment Epithelium of C57BL/6 Mice: S. Sukseree, et al.; PLoS One 11, e0161640 (2016), Application(s): Western blot analysis, RPE cell sheets, Abstract; Full Text
Autophagy limits proliferation and glycolytic metabolism in acute myeloid leukemia: A.S. Watson, et al.; Cell Death Discov. 1, 15008 (2015), Application(s): Immunohistochemistry, Full Text
Partial rescue of some features of Huntington Disease in the genetic absence of caspase-6 in YAC128 mice: B.K. Wong, et al.; Neurobiol. Dis. 76C, 24 (2015), Application(s): Western Blotting, Abstract;
Potassium Bisperoxo (1,10-phenanthroline) Oxovanadate (bpV(phen)) Induces Apoptosis and Pyroptosis and Disrupts the P62-HDAC6 Interaction to Suppress the Acetylated Microtubule-dependent Degradation of Autophagosomes: Q. Chen, et al.; J. Biol. Chem. 290, 26051 (2015), Abstract; Full Text
Regulated degradation of Chk1 by chaperone-mediated autophagy in response to DNA damage: C. Park, et al.; Nat. Commun. 6, 6823 (2015), Application(s): Western Blot, Abstract;
Time-Point Dependent Activation of Autophagy and the UPS in SOD1G93A Mice Skeletal Muscle: S. Olivan, et al. ; PLoS One 10, e0134830 (2015), Application(s): Immunoblot analysis, Abstract; Full Text
Autophagy inhibitor LRPPRC suppresses mitophagy through interaction with mitophagy initiator Parkin: J. Zou, et al.; PLoS One 9, e94903 (2014), Application(s): WB of mouse cells, Abstract; Full Text
Hyperactivation of mammalian target of rapamycin complex 1 (mTORC1) promotes breast cancer progression through enhancing glucose starvation-induced autophagy and Akt signaling: Y. Chen, et al.; J. Biol. Chem. 289, 1164 (2014), Application(s): WB of mouse tumor cells, Abstract;
p62/Sequestosome 1 Regulates Aggresome Formation of Pathogenic Ataxin-3 with Expanded Polyglutamine: L. Zhou, et al.; Int. J. Mol. Sci. 15, 14997 (2014), Application(s): WB and Fluoresecent Microscopy of human embryonic kidney cells (HEK293), Abstract; Full Text
Angiotensin-(1-7) inhibits autophagy in the brain of spontaneously hypertensive rats: T. Jiang, et al.; Pharmacol. Res. 71, 61 (2013), Application(s): WB of rat brain tissue, Abstract;
Autophagy deficiency by hepatic FIP200 deletion uncouples steatosis from liver injury in NAFLD: D. Ma, et al.; Mol. Endocrinol. 27, 1643 (2013), Application(s): WB of mouse hepatocytes, Abstract;
Autophagy Is Induced by UVA and Promotes Removal of Oxidized Phospholipids and Protein Aggregates in Epidermal Keratinocytes: Y. Zhao, et al.; J. Invest. Dermatol. 133, 1629 (2013), Application(s): WB of mouse keratinocytes, Abstract; Full Text
Balance between autophagic pathways preserves retinal homeostasis: N. Rodríguez-Muela, et al.; Aging Cell 3, 478 (2013), Application(s): IF, PCR, WB of mouse retina, Abstract; Full Text
Inhibition of LRRK2 kinase activity stimulates macroautophagy: C. Manzoni, et al.; Biochim. Biophys. Acta 1833, 2900 (2013), Application(s): WB of rat brain tissue, Abstract; Full Text
Inhibition of nonsense-mediated RNA decay activates autophagy: J. Wengrod, et al.; Mol. Cell. Biol. 33, 2128 (2013), Application(s): WB of human mRNA, Abstract; Full Text
Neuronal inactivation of peroxisome proliferator-activated receptor γ coactivator 1α (PGC-1α) protects mice from diet-induced obesity and leads to degenerative lesions: D. Ma, et al.; J. Biol. Chem. 285, 39087 (2013), Application(s): WB, qPCR of mouse tissue, Abstract; Full Text
Pale body-like inclusion formation and neurodegeneration following depletion of 26S proteasomes in mouse brain neurones are independent of α-synuclein: S.M. Paine, et al.; PLoS One 8, e54711 (2013), Application(s): Immunohistochemistry of mouse tissue, Abstract; Full Text
Pathogenic Parkinson's disease mutations across the functional domains of LRRK2 alter the autophagic/lysosomal response to starvation: C. Manzoni, et al.; Biochem. Biophys. Res. Commun. 441, 862 (2013), Application(s): WB, IC of mutated human primary fibroblasts, Abstract; Full Text
Proton-shuttling lichen compound usnic acid affects mitochondrial and lysosomal function in cancer cells: M. Bessadottir, et al.; PLoS One 7, e51296 (2013), Application(s): WB and IC of human cancer cells, Abstract; Full Text
Rho guanine nucleotide exchange factor is an NFL mRNA destabilizing factor that forms cytoplasmic inclusions in amyotrophic lateral sclerosis: C. Droppelmann, et al.; Neurobiol. Aging 34, 248 (2013), Application(s): IHC of mouse spinal cord tissue, Abstract;
Analysis of macroautophagy by immunohistochemistry: M. Rosenfeldt, et al.; Autophagy 8, 963 (2012), Application(s): WB, IHC of human and mouse tissues, Abstract; Full Text
Rapamycin prevents the mutant huntingtin-suppressed GLT-1 expression in cultured astrocytes: L. Chen, et al.; Acta Pharmacol. Sin. 33, 385 (2012), Application(s): WB of rat astrocytes , Abstract; Full Text
The bacterial and cellular determinants controlling the recruitment of mTOR to the Salmonella-containing vacuole: I. Tattoli, et al.; Biol. Open 1, 1215 (2012), Application(s): WB, IF, qPCR of bacteria, Abstract; Full Text
Temporal orchestration of circadian autophagy rhythm by C/EBPβ: D. Ma, et al.; EMBO J. 30, 4642 (2011), Application(s): WB of mouse tissue, Abstract; Full Text

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