Product Details
| Alternative Name: | SQSTM1, Sequestosome I |
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| Host: | Rabbit |
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| Immunogen: | Synthetic peptide corresponding to aa 387-436 human p62. |
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| UniProt ID: | Q13501 |
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| Source: | Purified from rabbit serum. |
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| Species reactivity: | Human
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| Applications: | ELISA, IF, IHC, WB
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| Recommended Dilutions/Conditions: | Immunohistochemistry (1:1,000)
Western Blot (1:1,000)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application. |
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| Purity Detail: | Peptide affinity purified. |
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| Formulation: | Liquid. In PBS containing 0.09% sodium azide. |
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| Handling: | Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C. |
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| Shipping: | Blue Ice |
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| Long Term Storage: | -20°C |
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| Scientific Background: | P62, also known as Sequestosome I, is a 62kDa, 440 amino acid protein, initially identified as a ligand of the SH2 domain of p56lck, now known to be expressed in many tissues. In addition to TRAF6, PEST and zinc finger motifs, p62 has a C-terminal ubiquitin binding association (UBA) domain with an affinity for multi-ubiquitin chains, and it is considered to serve as a scaffold protein, capable of binding to multiple signalling molecules and uniting receptor-mediated signalling events with ubiquitinylation. Elevated levels of p62 have been reported in breast tumours and in alcoholic liver disease where p62 has been shown to be involved in the formation of Mallory bodies. Several mutations in the p62 UBA domain have been identified and the etiology of Paget’s disease of bone has been linked to one such mutation. Kuusisto and colleagues have demonstrated that p62 is also present in elevated levels in the hallmark inclusions found in various neurodegenerative conditions, including tauopathies (Alzheimer’s disease, Picks disease, and frontotemporal dementia) and synucleinopathies (Parkinson’s disease, dementia with Lewy body disease and multiple system atrophy). In recent years ubiquitin immunostaining has been used to provide adjunct information for neuropathological diagnosis, but it is becoming evident that p62 may be an even more reliable marker of neurodegenerative disease inclusion detection than tau, alpha-synuclein or ubiquitin immunostaining. |
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| Technical Info/Product Notes: | Cited samples:
For an overview on cited samples please click here. |
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| Regulatory Status: | RUO - Research Use Only |
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Immunohistochemistry analysis of p62 immunoreactivity is present in neurons of the hippocampus of an Alzheimer patient. Note the intense reaction in the neurofibrillary tangles (dilution 1:1000).
Micrograph courtesy of Professor Fred van Leeuwen, University of Maastricht, The Netherlands.
Western blot analysis of wild type p62, GST-tagged (human, recombinant) bound to: (1) glutathione-agarose and (3) ubiquitin-agarose (2: agarose control). Bound species were analysed by PAGE followed by blotting on to PVDF and probing with Prod. No. BML-PW9860.
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SPRED2 deficiency elicits cardiac arrhythmias and premature death via impaired autophagy: M. Ullrich, et al.; J. Mol. Cell. Cardiol.
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Elevated Peripheral Myelin Protein 22, Reduced Mitotic Potential, and Proteasome Impairment in Dermal Fibroblasts from Charcot-Marie-Tooth Disease Type 1A Patients: S. Lee, et al.; Am. J. Pathol.
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Filamentous Aggregation of Sequestosome-1/p62 in Brain Neurons and Neuroepithelial Cells upon Tyr-Cre-Mediated Deletion of the Autophagy Gene Atg7: S. Sukseree, et al.; Mol. Neurobiol.
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Homozygous TBC1 domain-containing kinase (TBCK) mutation causes a novel lysosomal storage disease - a new type of neuronal ceroid lipofuscinosis (CLN15)?: S. Beck-Wodl, et al.; Acta Neuropathol. Commun.
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Defects in MAP1S-mediated autophagy cause reduction in mouse lifespans especially when fibronectin is overexpressed: W. Li, et al.; Aging Cell
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Protein degradation in a LAMP-2 deficient B-lymphoblastoid cell line from a patient with Danon disease: R. Sanchez-Lanzas, et al.; Biochim. Biophys. Acta
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Partial rescue of some features of Huntington Disease in the genetic absence of caspase-6 in YAC128 mice: B.K. Wong, et al.; Neurobiol. Dis.
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Potassium Bisperoxo (1,10-phenanthroline) Oxovanadate (bpV(phen)) Induces Apoptosis and Pyroptosis and Disrupts the P62-HDAC6 Interaction to Suppress the Acetylated Microtubule-dependent Degradation of Autophagosomes: Q. Chen, et al.; J. Biol. Chem.
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Regulation of endoplasmic reticulum turnover by selective autophagy: A. Khaminets, et al.; Nature
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p62/Sequestosome 1 Regulates Aggresome Formation of Pathogenic Ataxin-3 with Expanded Polyglutamine: L. Zhou, et al.; Int. J. Mol. Sci.
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Balance between autophagic pathways preserves retinal homeostasis: N. Rodríguez-Muela, et al.; Aging Cell
3, 478 (2013),
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Full Text
Bcl-2-dependent upregulation of autophagy by sequestosome 1/p62 in vitro: L. Zhou, et al.; Acta Pharmacol.
34, 651 (2013),
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Pale body-like inclusion formation and neurodegeneration following depletion of 26S proteasomes in mouse brain neurones are independent of α-synuclein: S.M. Paine, et al.; PLoS One
8, e54711 (2013),
Application(s): Immunohistochemistry of mouse tissue,
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Full Text
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441, 862 (2013),
Application(s): WB, IC of mutated human primary fibroblasts,
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7, e51296 (2013),
Application(s): WB and IC of human cancer cells,
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Full Text
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34, 248 (2013),
Application(s): IHC of mouse spinal cord tissue,
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Analysis of macroautophagy by immunohistochemistry: M. Rosenfeldt, et al.; Autophagy
8, 963 (2012),
Application(s): WB, IHC of human and mouse tissues,
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Full Text
Autophagy in the thymic epithelium is dispensable for the development of self-tolerance in a novel mouse model: S. Sukseree, et al.; PLoS One
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Full Text
Autophagy releases lipid that promotes fibrogenesis by activated hepatic stellate cells in mice and in human tissues: V. Hernadnez-Gea, et al.; Gastroenterology
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521, 130 (2012),
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Rapamycin prevents the mutant huntingtin-suppressed GLT-1 expression in cultured astrocytes: L. Chen, et al.; Acta Pharmacol. Sin.
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Application(s): WB, IF, qPCR of bacteria,
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Full Text
Temporal orchestration of circadian autophagy rhythm by C/EBPβ: D. Ma, et al.; EMBO J.
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