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Ub+1 polyclonal antibody

 
BML-PW9780-0025 25 µl 100.00 USD
 
BML-PW9780-0100 100 µl 320.00 USD
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Product Specification

Alternative Name:Ubiquitin+1
 
Host:Rabbit
 
Immunogen:Synthetic peptide corresponding to aa 76-95 of human Ub+1.
 
UniProt ID:P0CG47 (UBB), P0CG48 (UBC), P62979 (RPS27A), P62987 (UBA52)
 
Species reactivity:Human
 
Applications:IHC, WB
 
Recommended Dilutions/Conditions:Western Blot (1:1,000)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Formulation:Liquid. Antiserum containing 0.1% sodium azide.
 
Use/Stability:Store unopened vial at -20°C until required for use. The use of high quality 'antiserum-grade' plastic or glass vials is recommended.. Dilute to working strength with phosphate buffered saline pH 7.2-7.4 and 1% normal goat serum (if a goat anti-rabbit IgG linker antibody is to be used). Store diluted antibody at 2-4°C (do not freeze) and use within 1 month.
 
Handling:Do not freeze. After opening, prepare aliquots and store at -20°C.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Scientific Background:Ub+1 is a novel form of ubiquitin that has been selectively observed in the brains of Alzheimer's patients, including those with nonfamilial Alzheimer's disease. Ub+1 is an efficient substrate for polyubiquitinylation in vitro and in transfected human cells. The resulting polyubiquitin chains are refractory to disassembly by deubiquitinating enzymes and potently inhibit the degradation of a polyubiquitinylated substrate by purified 26S proteasomes. Thus, expression of Ub+1 in aging brain could result in dominant inhibition of the Ub-proteasome system, leading to neuropathologic consequences.

Expression of Ub+1 occurs through a phenomenon known as molecular misreading, in which non-DNA-encoded dinucleotide deletion(s) are found in mRNA within or adjacent to GAGAG motifs. A mechanism by which Ub+1 expression could lead to dominant inhibition of the Ub-proteasome system has been demonstrated.
 
bml-pw9780
Figure: Western blot analysis of Ub+1 (UW8790, 1µg) after SDS- PAGE followed by blotting onto PVDF and probing with PW780 with [lane (a)] and without [lane (b)] peptide blockade. Antibody dilution 1:1000 using ECL procedure.
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General Literature References

Inhibition of the ubiquitin-proteasome system in Alzheimer’s disease: Y. A. Lam et al.; PNAS 97, 9902 (2000), Abstract;
Frameshift mutants of beta amyloid precursor protein and ubiquitin-B in Alzheimer’s and Down patients: F. W. Van Leeuwen et al.; Science 279, 242 (1998), Abstract;
Mutations in RNA: a first example of molecular misreading in Alzheimer’s disease: F. W. Van Leeuwen et al.; Trends Neurosci. 21, 331 (1998), Abstract;
Age-related development of a heterozygous phenotype in solitary neurons of the homozygous Brattleboro rat: F. W. Van Leeuwen et al.; PNAS 86, 6417 (1989), Abstract;

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