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Immunoproteasome 20S (human), (purified)

 
BML-PW9645-0050 50 µg 482.00 USD
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Product Specification

Source:Isolated from human spleen.
 
Formulation:Liquid. In TEAD buffer (20mM TRIS/HCl, 1mM sodium azide, 1mM DTT, pH 7.4). Contains 50% glycerol.
 
Purity Detail:Highly purified. All starting material has been tested and found to be negative for hepatitis B surface antigen, human immunodeficiency virus type 1 antigens, and antibodies against human immunodeficiency viruses type 1 and 2, and hepatitis C virus.
 
Shipping:Shipped on Dry Ice
 
Long Term Storage:-80°C
 
Use/Stability:Once thawed the material can be stored at +4°C for up to 3 months, or long term at -20°C.
 
Handling:Avoid freeze/thaw cycles.
 
Scientific Background:The eukaryotic proteasome contains 7α-type and 7 β-type subunits. Upon stimulation with IFN-γ, the three active β subunits, β1 (Y), β2 (Z) and β5 (X) are exchanged to their immunocounterparts, namely β1i (LMP-2), β2i (MECL-1) and β5i (LMP-7). This results in a change in proteasomal substrate specificity. Some groups have reported increases in chymotrypsin-like and trypsin-like activities while others have reported no changes or even decreased activities after such treatment.
 
Immunoproteasome 20S (human), (purified) Western blot
Western blot analysis of Immunoproteasome 20S (human), (purified) (Prod. No. BML-PW9645) probed with anti-Syntaxin 3 antibody (ab4113) and anti-Proteasome 20S LMP2 antibody (ab42987).
Immunoproteasome 20S (human), (purified) SDS-PAGE
Coomassie stained gel after SDS-PAGE (12.5% acrylamide). Lane (a): molecular weight markers; lane (b) 20S immunoproteosome, spleen-derived (prod.no. BML-PW9645); lane (c): 20S proteosome, erythrocyte-derived (PW8720).
Immunoproteasome 20S (human), (purified) activity
All activities were measured by use of 200µM substrate concentration (final concentration) all dissolved in TEAD buffer. Test system: 20µl enzyme solution (640ng); 20µl substrate solution (incubation at 37°C); 200µl stop solution (100mM sodium chloroacetate dissolved in 30mM Na-acetate, 70mM acetic acid, pH 4.3). Fluorescence was measured in a microplate fluorimeter at 355nm excitation and 460nm emission.
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Immunoproteasome 20S (human), (purified) Western blot Immunoproteasome 20S (human), (purified) SDS-PAGE Immunoproteasome 20S (human), (purified) activity

Product Literature References

Induction of the Immunoproteasome Subunit Lmp7 Links Proteostasis and Immunity in α-Synuclein Aggregation Disorders: S. Ugras, et al.; EBioMedicine 31, 307 (2018), Abstract; Full Text
The presence of prolines in the flanking region of an immunodominant HIV-2 gag epitope influences the quality and quantity of the epitope generated: S. Jallow, et al.; Eur. J. Immunol. 45, 2232 (2015), Abstract; Full Text

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