Online Purchasing Account You are logged on as Guest. LoginRegister a New AccountShopping cart (Empty)
United States 

Proteasome 26S (human), (purified)

High integrity preparation for use in proteasome research.
 
BML-PW9310-0050 50 µg 361.00 USD
Do you need bulk/larger quantities?
 
Highly purified preparation of ‘26S’ proteasomes useful for carrying out in vitro protein degradation studies with suitably ubiquitinylated protein substrates.

Product Specification

Source:Isolated from human erythrocytes. Consists of a high purity mixture of ‘26S’ proteasomes singly (26S) and doubly (30S) capped with 19S regulatory subunit complexes in the ratio of 40% single cap : 60% double capped at the time of preparation.
 
Formulation:Suspended in TSD buffer (50μg in 10mM TRIS, containing 25mM potassium chloride, 1.1mM magnesium chloride, 0.1mM ethylenediaminetetraacetic acid, 1mM dithiothreitol, 1mM sodium azide, 2mM ATP, pH 7.0, and 35% glycerol).
 
Shipping:Shipped on Dry Ice
 
Long Term Storage:-80°C
 
Use/Stability:When ready for use the enzyme should be thawed by standing on ice. If the enzymatic acitivity of the 26S proteasome is to be measured, it should be used immediately after thawing since the enzyme complex is labile. After dissociaton of the 26S complex the 20S proteasome activity is relatively stable.
 
bml-pw9310
A - Typical results from substrate overlay (carried out in buffer containing ATP and an ATP-regenerating system) and Coomassie staining of non denaturing gel of products isolated according to the purification shown alongside. Protein concentration is ~15-20µg per lane.. B – Coomassie staining of denaturing gel showing presence of proteasomal subunits.
bml-pw9130 a

A typical example of a sucrose density gradient separation and purification of 20S, 26S and 30S proteasome derived from human erythrocytes together with electron micrographs of the individual complexes. The protein concentration used for micrography is best at ~2.5µg/mL.

Please mouse over
bml-pw9310 bml-pw9130 a

Product Literature References

Dietary apigenin potentiates the inhibitory effect of interferon-α on cancer cell viability through inhibition of 26S proteasome-mediated interferon receptor degradation: S. Li, et al.; Food Nutr. Res. 60, 31288 (2016), Application(s): Cell culture and cell-based assay, Abstract; Full Text
Reconstitution of hybrid proteasomes from purified PA700-20 S complexes and PA28alphabeta activator: ultrastructure and peptidase activities: F. Kopp et al.; J. Mol. Biol. 313, 465 (2001), Abstract;
Studies on the activation by ATP of the 26 S proteasome complex from rat skeletal muscle: B. Dahlmann et al.; Biochem. J. 309 , 195 (1995), Abstract;

Product Toolbox

PRODUCT RESOURCES

Datasheet
MSDS
Certificate of Analysis

RELATED PRODUCTS

By target:
Proteasome 26S
By biological activity:
Proteasome 26S Native protein
By catalog section:

PRODUCT SUPPORT

FAQs
Technical Service
Customer Service

Related Literature

Catalogs
Ubiquitin & UBL Signaling Catalog
Ubiquitin & UBL Signaling Catalog
Download as PDF

Product Flyers
Proteasome ELISA Kit
Proteasome ELISA Kit
Download as PDF

All new literature pieces

Recommend this page

 
For Research Use Only. Not for use in diagnostic procedures.
Keep in touch

©2017 Enzo Life Sciences, Inc.,