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Proteasome 20S β2 subunit monoclonal antibody (MCP165)

 
BML-PW9300-0025 25 µl 108.00 USD
 
BML-PW9300-0100 100 µl 334.00 USD
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Product Specification

Alternative Name:Proteasome subunit β type-7, Macropain chain Z
 
Clone:MCP165
 
Host:Mouse
 
Isotype:IgG1
 
Immunogen:Dinitrophenylated proteasomes.
 
UniProt ID:Q99436 (human), P70195 (mouse)
 
Gene/Protein Identifier:PSMB7 (gene name)
 
Species reactivity:Human, Mouse
 
Specificity:Recognizes the β2 subunit of the 20S proteasome.
 
Applications:IHC, WB
 
Recommended Dilutions/Conditions:Western Blot (1:1,000)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Application Notes:Immunoprecipitation: Does not immunoprecipitate intact proteasomes from HeLa cell preparations.
Immunohistochemistry: Has been shown to have applicability in immunohistochemical applications using human tissue.
Western blot: Single dimension SDS-PAGE of a HeLa cell lysate followed by Western blotting gives a single band with a relative molecular weight of ~28kDa.
 
Purity Detail:Protein G affinity purified.
 
Formulation:Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide.
 
Use/Stability:Store unopened vial at -20°C until required for use. Aliquot undiluted antibody into smaller volumes (not less than 10μl) prior to freezing if appropriate. The use of high quality ‘antiserum-grade’ plastic or glass vials is recommended. Store diluted antibody at 2-4°C (do not freeze) and use within 1 month.
 
Handling:Avoid freeze/thaw cycles.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Scientific Background:The hybridoma secreting the antibody to subunit β2 was generated by fusion of splenocytes from Balb/c mice which had received repeated immunization with dinitrophenylated proteasomes. The antibody (clone MCP165) has been characterised by one- and two dimensional Western blotting. The antibody has not been demonstrated to immunoprecipitate the native 20S proteasome. The immunoglobulin subclass is IgG1.
 
Technical Info/Product Notes:Various systems for the nomenclature of the proteasome subunits have been established. This may be a source of confusion as the system on UniProt differs from "standard" nomenclature as described in the literature. The UniProt ID and Gene Name will help to clearly identify the proteins.
 
BML-PW9300 WB
Western Blot Analysis of Proteasome 20S β2 subunit, mAb (MCP165), (Prod. No. BML-PW9300): Lane 1: MW Marker, Lane 2: HeLa Cell Lysate (Prod. No. ADI-LYC-HL100)
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BML-PW9300 WB

Product Literature References

SOD1 deficiency decreases proteasomal function, leading to the accumulation of ubiquitinated proteins in erythrocytes: T. Homma, et al.; Arch. Biochem. Biophys. 583, 65 (2015), Application(s): Immunoblotting, Abstract;

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