Online Purchasing Account You are logged on as Guest. Login Register a New Account Shopping cart (Empty)

Proteasome 19S Rpn10/S5a subunit monoclonal antibody (S5a-18)



BML-PW9250-0025	25 µl	169.00 USD

BML-PW9250-0100	100 µl	475.00 USD
Do you need bulk/larger quantities?

Product Details

Alternative Name:	26S proteasome non-ATPase regulatory subunit 4, Antisecretory factor 1, Multiubiquitin chain-binding protein

Clone:	S5a-18

Host:	Mouse

Isotype:	IgG1

Immunogen:	Recombinant full-length human Rpn10 protein.

UniProt ID:	P55036

Gene/Protein Identifier:	PSMD4 (gene name)

Source:	Purified from hybridoma tissue culture supernatant.

Species reactivity:	Human, Rat Bovine

Specificity:	Recognizes the Rpn10/S5a subunit of the 19S regulator complex.

Applications:	IP, WB

Recommended Dilutions/Conditions:	Western Blot (1:1,000, ECL) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application.

Formulation:	Liquid. In PBS containing 10mM sodium azide.

Handling:	Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.

Shipping:	Blue Ice

Long Term Storage:	-20°C

Regulatory Status:	RUO - Research Use Only

Proteasome 19S Rpn10/S5a subunit monoclonal antibody (S5a-18) Western blot

Figure 1. Western blot analysis of Proteasome 19S Rpn10/S5a subunit, mAb (S5a-18) (Prod. No. BML-PW9250): Lane 1: MW marker, Lane 2: 3T3 (Prod. No. ADI-LYC-3T100), Lane 3: L929, Lane 4: PC-12 (Prod. No. ADI-LYC-PC100), Lane 5: MDBK, Lane 6: HeLa S100 fraction (Prod. No. BML-SW8750).

Please mouse over

Product Literature References

New insights into host-parasite ubiquitin proteome dynamics in P. falciparum infected red blood cells using a TUBEs-MS approach: L. Mata-Cantero, et al.; J. Proteomics 139, 45 (2016), Application(s): Western blot, Abstract;

Bortezomib Amplifies Effect on Intracellular Proteasomes by Changing Proteasome Structure: D.S. Pitcher, et al.; EBioMedicine 2, 642 (2015), Application(s): Western Blot, Abstract;

Nuclear proteasomes carry a constitutive posttranslational modification which derails SDS-PAGE (but not CTAB-PAGE): D.S. Pitcher, et al.; Biochim. Biophys. Acta 1844, 2222 (2014), Abstract;

The degradation of p53 and its major E3 ligase Mdm2 is differentially dependent on the proteasomal ubiquitin receptor S5a: A. Sparks, et al.; Oncogene 33, 4685 (2014), Application(s): WB using human cell lysates, Abstract; Full Text