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Proteasome 19S Rpn10/S5a subunit monoclonal antibody (S5a-18)

 
BML-PW9250-0025 25 µl 118.00 USD
 
BML-PW9250-0100 100 µl 352.00 USD
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Replaces Prod. #: ALX-210-320

Product Specification

Alternative Name:26S proteasome non-ATPase regulatory subunit 4, Antisecretory factor 1, Multiubiquitin chain-binding protein
 
Clone:S5a-18
 
Host:Mouse
 
Isotype:IgG1
 
Immunogen:Recombinant full-length human Rpn10 protein.
 
UniProt ID:P55036
 
Species reactivity:Human, Rat
Bovine
 
Specificity:Recognizes the Rpn10/S5a subunit of the 19S regulator complex.
 
Applications:IP, WB
 
Recommended Dilutions/Conditions:Western Blot (1:1,000, ECL)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Formulation:Liquid. In PBS containing 10mM sodium azide.
 
Handling:Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
BML-PW9250 WB
Figure 1. Western blot analysis of Proteasome 19S Rpn10/S5a subunit, mAb (S5a-18) (Prod. No. BML-PW9250): Lane 1: MW marker, Lane 2: 3T3 (Prod. No. ADI-LYC-3T100), Lane 3: L929, Lane 4: PC-12 (Prod. No. ADI-LYC-PC100), Lane 5: MDBK, Lane 6: HeLa S100 fraction (Prod. No. BML-SW8750).
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BML-PW9250 WB

Product Literature References

New insights into host-parasite ubiquitin proteome dynamics in P. falciparum infected red blood cells using a TUBEs-MS approach: L. Mata-Cantero, et al.; J. Proteomics 139, 45 (2016), Application(s): Western blot, Abstract;
Bortezomib Amplifies Effect on Intracellular Proteasomes by Changing Proteasome Structure: D.S. Pitcher, et al.; EBioMedicine 2, 642 (2015), Application(s): Western Blot, Abstract;
The degradation of p53 and its major E3 ligase Mdm2 is differentially dependent on the proteasomal ubiquitin receptor S5a: A. Sparks, et al.; Oncogene 33, 4685 (2014), Application(s): WB using human cell lysates, Abstract; Full Text

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