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Proteasome 20S β5 subunit (human) polyclonal antibody

BML-PW8895-0025 25 µl 110.00 USD
BML-PW8895-0100 100 µl 352.00 USD
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Product Specification

Alternative Name:Proteasome subunit β type-5, Macropain ε chain, Proteasome chain 6, Proteasome subunit MB1, Proteasome subunit X
Immunogen:Synthetic peptide corresponding to aa 194-207 of human proteasome 20S (β5 subunit).
UniProt ID:P28074
Gene/Protein Identifier:PSMB5 (gene name)
Species reactivity:Human
Specificity:Recognizes human proteasome 20S β5 subunit.
Applications:IHC, WB
Formulation:Liquid. In PBS containing 0.09% sodium azide.
Handling:Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.
Shipping:Shipped on Blue Ice
Long Term Storage:-20°C
Technical Info/Product Notes:Various systems for the nomenclature of the proteasome subunits have been established. This may be a source of confusion as the system on UniProt differs from "standard" nomenclature as described in the literature. The UniProt ID and Gene Name will help to clearly identify the proteins.

Product Literature References

Involvement of the ubiquitin-proteasome system in the expression of extracellular matrix genes in retinal pigment epithelial cells: J.E. Ramos de Carvalho, et al.; Biochem. Biophys. Rep. 13, 83 (2018), Abstract;
Detection of active proteasome structures in brain extracts: proteasome features of August rat brain with violations in monoamine metabolism: P.A. Erokhov, et al.; Oncotarget 8, 70695 (2017), Abstract; Full Text
Circadian modulation of proteasome activity and accumulation of oxidized protein in human embryonic kidney HEK 293 cells and primary dermal fibroblasts: A. Desvergne, et al.; Free Radic. Biol. Med. 94, 195 (2016), Application(s): Western blot, Abstract;
Efficacy of panobinostat and marizomib in acute myeloid leukemia and bortezomib-resistant models: F.F. Corrales-Medina, et al.; Leuk. Res. 39, 371 (2015), Application(s): Western Blotting, Abstract;
Skeletal muscle myotubes in severe obesity exhibit altered ubiquitin-proteasome and autophagic/lysosomal proteolytic flux: L.M. Bollinger, et al.; Obesity (Silver Spring) 23, 1185 (2015), Application(s): Western Blot, Abstract; Full Text
SOD1 deficiency decreases proteasomal function, leading to the accumulation of ubiquitinated proteins in erythrocytes: T. Homma, et al.; Arch. Biochem. Biophys. 583, 65 (2015), Application(s): Immunoblotting, Abstract;
The degradation of p53 and its major E3 ligase Mdm2 is differentially dependent on the proteasomal ubiquitin receptor S5a: A. Sparks, et al.; Oncogene 33, 4685 (2014), Application(s): WB using human cell lysates, Abstract; Full Text
The small heat shock protein B8 (HSPB8) confers resistance to bortezomib by promoting autophagic removal of misfolded proteins in multiple myeloma cells: M. Hamouda, et al.; Oncotarget 5, 6252 (2014), Application(s): Analysis of velcade resistant multiple myeloma human cells by WB, Assay, Abstract; Full Text
Negative regulation of 26S proteasome stability via calpain-mediated cleavage of Rpn10 subunit upon mitochondrial dysfunction in neurons: Q. Huang, et al.; J. Biol. Chem. 288, 12161 (2013), Abstract;
Proteasome inhibitors block DNA repair and radiosensitize non-small cell lung cancer: K.R. Cron, et al.; PLoS One 8, e73710 (2013), Application(s): Western blot, Abstract; Full Text
Proteasome inhibitors protect the steatotic and non-steatotic liver graft against cold ischemia reperfusion injury: M.A. Zaouali, et al.; Exp. Mol. Pathol. 94, 352 (2013), Application(s): Western blot, Abstract;
RedOx status, proteasome and APEH: insights into anticancer mechanisms of t10, c12-conjugated linoleic acid isomer on A375 melanoma cells: P. Bergamo, et al.; PLoS One 8, e80900 (2013), Application(s): Western blot, Abstract; Full Text
A putative role for the immunoproteasome in the maintenance of pluripotency in human embryonic stem cells: S.P. Atkinson, et al.; Stem Cells 30, 1373 (2012), Application(s): Western blot, Abstract; Full Text
Bortezomib resistance in a myeloma cell line is associated to PSMβ5 overexpression and polyploidy: P. Balsas, et al.; Leuk. Res. 36, 212 (2012), Application(s): Western blot, Abstract;
Dynamics of the degradation of ubiquitinated proteins by proteasomes and autophagy: association with sequestosome 1/p62: N. Myeku, et al.; J. Biol. Chem. 286, 22426 (2011), Application(s): Western blot, Abstract; Full Text

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