Mono- and polyubiquitinylated conjugates, mAb (FK2)



BML-PW8810-0500	500 µg	360.00 USD

The hybridoma secreting the MAb to Mono- and Polyubiquitinylated Conjugates (FK2) (Prod. No. BML-PW8805) was generated by fusion of splenocytes from Balb/c mice which had received repeated immunisation with a crude preparation of polyubiquitinylated-lysozyme. MAb to Mono- and Polyubiquitinylated Conjugates (FK2) has been extensively characterised by one-dimensional Western blotting and has been shown to recognise K²⁹-, K⁴⁸-, and K⁶³-linked polyubiquitinylated and monoubiquitinylated proteins but not free ubiquitin.

Product Specification

Clone:	FK2

Host:	Mouse

Isotype:	IgG1

Immunogen:	Poly-ubiquitinylated lysozyme.

Application:	ELISA Immunohistochemistry Immunoprecipitation (Care must be taken as MAb to Mono- and Polyubiquitinylated Conjugates (FK2) demonstrates affinity for both free ubiquitin and multi-ubiquitinylated species when immobilised) Western Blot (1:100-1:1000) Optimal conditions must be determined individually for each application.

Specificity:	Recognizes mono- and polyubiquitinylated conjugates. Species independent. Does not cross-react with free ubiquitin.

Purity Detail:	Purified.

Formulation:	Liquid. In PBS containing 0.1% sodium azide.

Handling:	Avoid freeze/thaw cycles. Store unopened vial at -20°C until required for use. Store diluted antibody at 2-4°C and use within 1 month.

Long Term Storage:	-20°C

Figure 1: Western blot of multi-ubiquitin chains using MAb to Polyubiquitinylated Conjugates (FK1) (Prod. No. BML-PW8805) (lanes A-C) and MAb to Mono- and Polyubiquitinylated Conjugates (FK2) (Prod. No. BML-PW8810) (lanes D-F).

Lanes A & D: K⁴⁸-linked chains.Lanes B & E: K⁴⁹-linked chains. Lanes C & F: K⁶³-linked chains.

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Product Literature References

Autophagy inhibition promotes defective neosynthesized proteins storage in ALIS, and induces redirection toward proteasome processing and MHCI-restricted presentation: T. Wenger et al; Autophagy 8, 350 (2012), Abstract;

Heterologous SUMO-2/3-Ubiquitin Chains Optimize IκBα Degradation and NF-κB Activity: F. Aillet, et al.; PLoS One 7, e51672 (2012), Abstract; Full Text

The proteasomal de-ubiquitinating enzyme POH1 promotes the double-strand DNA break response: L.R. Butler et al; EMBO 31, 3918 (2012), Abstract;

Loss of hrs in the central nervous system causes accumulation of ubiquitinated proteins and neurodegeneration: K. Tamai, et al.; Am. J. Pathol. 173, 1806 (2008), Abstract;

Large-scale analysis of the human ubiquitin-related proteome : Matsumoto, M. et al. ; Proteomics 5, 4145 (2005), Abstract;

Chemotropic responses of retinal growth cones mediated by rapid local protein synthesis and degradation: D.S. Campbell et al.; Neuron 32, 1013 (2001), Abstract;

Isolation of ubiquitin-E2 (ubiquitin conjugating enzyme complexes from erythroleukaemia cells using immunoaffinity techniques: K. Takada et al.; Biochem. J. 356, 199 (2001), Abstract; Full Text

MHC Class I ubiquitination by a viral PHD/LAP finger protein: J.N. Boname et al.; Immunity 15, 627 (2001), Abstract;

Dynamics of ubiquitin conjugation during heat-shock response revealed by using a monoclonal antibody specific to multi-ubiquitin chains: M. Fujimuro, et al.; Eur. J. Biochem. 249, 427 (1997), Abstract;

Serum concentrations of free ubiquitin and multiubiquitin chains: K. Takada et al.; Clin. Chem. 43, 1188 (1997), Abstract; Full Text

Immunoassay for the quantification of intracellular multi-ubiquitin chains: K. Takada, et al.; Eur. J. Biochem. 233, 42 (1995), Abstract;

Production and characterization of monoclonal antibodies specific to multi-ubiquitin chains of polyubiquitinated proteins: M. Fujimuro, et al.; FEBS Lett. 349, 173 (1994), Abstract;