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Mono- and polyubiquitinylated conjugates, mAb (FK2)

Gold standard antibody for detection of ubiquitinylated proteins.
 
BML-PW8810-0100 100 µg 90.00 USD
 
BML-PW8810-0500 500 µg 300.00 USD
 
  • Detects K29-, K48-, and K63-linked mono- and polyubiquitinylated proteins
  • Most cited multi-ubiquitin antibody with over 500 citations
  • Validated for WB, IP, IHC, and ELISA applications
  • Available as HRP, biotin, ATTO 488, and fluorescein labeled conjugates
The hybridoma secreting the MAb to Mono- and Polyubiquitinylated Conjugates (FK2) (Prod. No. BML-PW8805) was generated by fusion of splenocytes from Balb/c mice which had received repeated immunisation with a crude preparation of polyubiquitinylated-lysozyme. MAb to Mono- and Polyubiquitinylated Conjugates (FK2) has been extensively characterised by one-dimensional Western blotting and has been shown to recognise polyubiquitinylated and monoubiquitinylated proteins regardless of linkage location but not free ubiquitin.

Product Specification

Clone:FK2
 
Host:Mouse
 
Isotype:IgG1
 
Immunogen:Poly-ubiquitinylated lysozyme.
 
Species reactivity:Species independent
 
Specificity:Recognizes mono- and polyubiquitinylated conjugates.
 
Crossreactivity:Does not cross-react with free ubiquitin.
 
Applications:ELISA, IHC, IP, WB
 
Recommended Dilutions/Conditions:Immunoprecipitation (care must be taken as MAb to Mono- and polyubiquitinylated conjugates (FK2) demonstrates affinity for both free ubiquitin and multi-ubiquitinylated species when immobilized)
Western Blot (1:100-1:1,000)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Purity Detail:Purified.
 
Formulation:Liquid. In PBS containing 0.1% sodium azide.
 
Handling:Store unopened vial at -20°C until required for use. Store diluted antibody at 2-4°C and use within 1 month. Avoid freeze/thaw cycles.
 
Long Term Storage:-20°C
 
bml-pw8810
Figure 1: Western blot of multi-ubiquitin chains using MAb to Polyubiquitinylated Conjugates (FK1) (Prod. No. BML-PW8805) (lanes A-C) and MAb to Mono- and Polyubiquitinylated Conjugates (FK2) (Prod. No. BML-PW8810) (lanes D-F).

Lanes A & D: K48-linked chains.Lanes B & E: K29-linked chains. Lanes C & F: K63-linked chains.

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bml-pw8810

Product Literature References

Autophagy inhibition promotes defective neosynthesized proteins storage in ALIS, and induces redirection toward proteasome processing and MHCI-restricted presentation: T. Wenger et al; Autophagy 8, 350 (2012), Abstract;
Heterologous SUMO-2/3-Ubiquitin Chains Optimize IκBα Degradation and NF-κB Activity: F. Aillet, et al.; PLoS One 7, e51672 (2012), Abstract; Full Text
The proteasomal de-ubiquitinating enzyme POH1 promotes the double-strand DNA break response: L.R. Butler et al; EMBO 31, 3918 (2012), Abstract;
Dynamics of the degradation of ubiquitinated proteins by proteasomes and autophagy: association with sequestosome 1/p62: N. Myeku, et al.; J. Biol. Chem. 286, 22426 (2011), Application(s): Western blot, Abstract; Full Text
Immunoreactivity to Lys63-linked polyubiquitin is a feature of neurodegeneration: S. Paine, et al.; Neurosci. Lett. 460, 205 (2009), Application(s): Immunohistochemistry of mouse hippocampus, Abstract;
Loss of hrs in the central nervous system causes accumulation of ubiquitinated proteins and neurodegeneration: K. Tamai, et al.; Am. J. Pathol. 173, 1806 (2008), Abstract;
Large-scale analysis of the human ubiquitin-related proteome : Matsumoto, M. et al. ; Proteomics 5, 4145 (2005), Abstract;
Chemotropic responses of retinal growth cones mediated by rapid local protein synthesis and degradation: D.S. Campbell et al.; Neuron 32, 1013 (2001), Abstract;
Isolation of ubiquitin-E2 (ubiquitin conjugating enzyme complexes from erythroleukaemia cells using immunoaffinity techniques: K. Takada et al.; Biochem. J. 356, 199 (2001), Abstract; Full Text
MHC Class I ubiquitination by a viral PHD/LAP finger protein: J.N. Boname et al.; Immunity 15, 627 (2001), Abstract;
Dynamics of ubiquitin conjugation during heat-shock response revealed by using a monoclonal antibody specific to multi-ubiquitin chains: M. Fujimuro, et al.; Eur. J. Biochem. 249, 427 (1997), Abstract;
Serum concentrations of free ubiquitin and multiubiquitin chains: K. Takada et al.; Clin. Chem. 43, 1188 (1997), Abstract; Full Text
Immunoassay for the quantification of intracellular multi-ubiquitin chains: K. Takada, et al.; Eur. J. Biochem. 233, 42 (1995), Abstract;
Production and characterization of monoclonal antibodies specific to multi-ubiquitin chains of polyubiquitinated proteins: M. Fujimuro, et al.; FEBS Lett. 349, 173 (1994), Abstract;

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