Polyubiquitinylated conjugates, mAb (FK1)

Widely used antibody for detection of polyubiquitinylated proteins.



BML-PW8805-0500	500 µg	323.00 USD

The hybridoma secreting MAb to Polyubiquitinylated Conjugates (FK1) (Prod. No. BML-PW8805) was generated by fusion of splenocytes from Balb/c mice which had received repeated immunisation with a crude preparation of polyubiquitinylated-lysozyme. MAb to Polyubiquitinylated Conjugates (FK1) has been extensively characterised by one-dimensional Western blotting and has been shown to recognise only polyubiquitinylated proteins and not monoubiquitinylated proteins or free ubiquitin.

Product Specification

Purity Detail:	Purified.

Formulation:	Liquid. In PBS containing 0.1% sodium azide.

Clone:	FK1

Isotype:	IgM

Immunogen:	Poly-ubiquitinylated lysozyme.

Source/Host:	From mouse

Specificity:	Recognizes polyubiquitinylated conjugates. Species independent. Does not cross-react with mono-ubiquitinylated proteins or free ubiquitin.

Application:	ELISA Immunohistochemistry Western Blot: (1:1’000) Optimal conditions must be determined individually for each application.

Long Term Storage:	+4°C

Handling:	Do not freeze. Store unopened vial at 4°C until required for use. Store diluted antibody at 2-4°C and use within 1 month.

Background / Technical Information:	Do not use milk as blocking solution. Use 1% BSA in PBS or TBS-Tween instead.Please click here for the comprehensive product datasheet.

Figure 1: Western blot of multi-ubiquitin chains using MAb to Polyubiquitinylated Conjugates (FK1) (Prod. No. BML-PW8805) (lanes A-C) and MAb to Mono- and Polyubiquitinylated Conjugates (FK2) (Prod. No. BML-PW8810) (lanes D-F).

Lanes A & D: K⁴⁸-linked chains.Lanes B & E: K⁴⁹-linked chains. Lanes C & F: K⁶³-linked chains.

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Product Literature References

PINK1/Parkin-mediated mitophagy is dependent on VDAC1 and p62/SQSTM1: S. Geisler, et al.; Nat. Cell Biol. 12, 119 (2010), Abstract;

Multiple monoubiquitination of RTKs is sufficient for their endocytosis and degradation: K. Haglund et al.; Nat. Cell. Biol. 5, 461 (2003), Abstract;

The FMR1 CGG repeat mouse displays ubiquitin-positive intranuclear neuronal inclusions; implications for the cerebellar tremor/ataxia syndrome: R. Willemsen et al.; Hum. Mol. Genet. 12, 949 (2003), Abstract;

Mammalian class E vps proteins recognize ubiquitin and act in the removal of endosomal protein-ubiquitin conjugates: N. Bishop et al.; J. Cell. Biol. 157, 91 (2002), Abstract;

PI31 is a modulator of proteasome formation and antigen processing: D. M. W. Zaiss; PNAS 99, 14344 (2002), Abstract;

Transient aggregation of ubiquitinated proteins during dendritic cell maturation: H. Lelouard et al.; Nature 417, 177 (2002), Abstract;

Dynamics of ubiquitin conjugation during heat-shock response revealed by using a monoclonal antibody specific to multi-ubiquitin chains: M. Fujimuro et al.; Eur. J. Biochem. 249, 427 (1997), Abstract; Full Text

Serum concentrations of free ubiquitin and multiubiquitin chains: K. Takada et al.; Clin. Chem. 43, 1188 (1997), Abstract; Full Text

Immunoassay for the quantification of intracellular multiubiquitin chains: K. Takada et al.; Eur. J. Biochem. 233, 42 (1995), Abstract; Full Text

Production and characterisation of monoclonal antibodies specific to multiubiquitin chains of polyubiquitinated proteins. : M. Fujimuro et al.; FEBS Letts. 349, 173 (1994), Abstract;