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Proteasome 20S (Saccharomyces cerevisiae)

BML-PW8775-0050 50 µg 245.00 USD

Product Specification

Source:Purified from Saccharomyces cerevisiae.
Formulation:Liquid. In 40mM TRIS-HCl, pH 7.5, containing 10mM MgCl2, 250mM sodium chloride and 20% glycerol.
Purity:≥90% (SDS-PAGE)
Shipping:Shipped on Blue Ice
Short Term Storage:+4°C
Long Term Storage:-20°C
Use/Stability:Stable at +4°C for a week, at -20°C for 6 months after receipt.
Handling:Avoid -80°C.
Scientific Background:The 20S proteasome is the catalytic core of the 26S proteasome, the central protease of the ubiquitin pathway of protein degradation. Unlike the 26S proteasome, which degrades proteins in an ATP-dependent manner, the 20S proteasome is ATP-independent and only degrades entirely unfolded polypeptides.
BML-PW8775 activity assay
Figure 1. Proteasome 20S (Saccharomyces cerevisiae) activity/inhibition assay. 0.5μg Proteasome 20S (Saccharomyces cerevisiae) (Prod. No. BML-PW8775) in activity assay buffer (50mM Tris-HCl, pH 7.5, 0.02% SDS, 1mM MgCl2, 50mM NaCl) with or without Lactacystin (Prod. No. BML-PI104) for 30 min pre-incubation. Substrate Suc-Leu-Leu-Val-Tyr-AMC (Prod. No. BML-P802) was then added to 0.5mM final concentration. Release of fluorophore was measured at ex360/em460 at every 0.5hr time points.
Figure 2. Silver stained gel after SDS-PAGE. Lane (a): 1600ng 20S proteasome; (b) 800ng 20S proteasome; (c) 400 20S proteasome; (d) 200ng 20S proteasome; (e) 100ng 20S proteasome; (f) 50ng 20S proteasome; (g) molecular weight markers (14, 21, 31, 36, 55, 66, 97, 116, 200kDa).
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BML-PW8775 activity assay BML-PW8775 SDS-PAGE

Product Literature References

Small Molecule Modulation of Proteasome Assembly: E. Njomen, et al.; Biochemistry 57, 4214 (2018), Abstract; Full Text
Marchantin M: a novel inhibitor of proteasome induces autophagic cell death in prostate cancer cells: H. Jiang, et al.; Cell Death Dis. 4, e761 (2013), Application(s): Kinetics assay, Abstract; Full Text

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