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Proteasome 20S (human), (purified)

High integrity preparation for use in proteasome research.
 
BML-PW8720-0050 50 µg 185.00 USD
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Replaces Prod. #: ALX-202-047

Product Specification

Source:Isolated and purified from human erythrocytes.
 
Concentration:1.0mg/ml
 
Formulation:Liquid. In 20mM TRIS-HCl, pH 7.2, containing 1mM EDTA, 1mM DTT, 1mM sodium azide and 50% glycerol.
 
Shipping:Shipped on Dry Ice
 
Long Term Storage:-80°C
 
Use/Stability:20S Proteasome remains relatively stable and active so long as repetitive freeze-thaw cycles are avoided. It has been demonstrated that 20S proteasome undergoes only slow loss of activity when stored at 4°C for up to six months. If it is to be used on a regular basis, it is recommended that the material should be stored between 0°C-4°C during the duration of use. For longer term storage the material may be dispensed as single use aliquots and stored at -80°C.
 
Handling:Avoid freeze/thaw cycles.
 
Scientific Background:The 20S proteasome is the catalytic core of the 26S proteasome, the central protease of the ubiquitin pathway of protein degradation. Unlike the 26S proteasome, which degrades proteins in an ATP-dependent manner, the 20S proteasome is ATP-independent and only degrades entirely unfolded polypeptides.
 

Product Literature References

Nano-liquid chromatography-orbitrap MS -based quantitative proteomics reveals differences between the mechanisms of action of carnosic acid and carnosol in colon cancer cells: A. Valdés, et al.; Mol. Cell. Proteomics 16, 8 (2017), Abstract; Full Text
An informatic framework for decoding protein complexes by top-down mass spectrometry: O.S. Skinner, et al.; Nat. Methods. 13, 237 (2016), Abstract;
Oxidation and interaction of DJ-1 with 20S proteasome in the erythrocytes of early stage Parkinson’s disease patients: Y. Saito, et al.; Sci. Rep. 6, 30793 (2016), Application(s): Interaction analysis with oxidized DJ-1 recombinant protein, Abstract; Full Text
Total Sample Conditioning and Preparation of Nanoliter Volumes for Electron Microscopy: S.A. Arnold, et al.; ACS Nano 10, 4981 (2016), Application(s): Single-Particle test samples, Abstract; Full Text
PiZ Mouse Liver Accumulates Polyubiquitin Conjugates That Associate with Catalytically Active 26S Proteasomes: C.J. Haddock, et al.; PLoS One 9, e106371 (2014), Application(s): EM Analysis, Abstract; Full Text
Quinone-induced protein handling changes: implications for major protein handling systems in quinone-mediated toxicity: R. Xiong, et al.; Toxicol. Appl. Pharmacol. 280, 285 (2014), Application(s): Assay, Abstract;
Differential Roles of Proteasome and Immunoproteasome Regulators Pa28αβ, Pa28γ and Pa200 in the Degradation of Oxidized Proteins: A.M. Pickering, et al.; Arch. Biochem. Biophys. 523, 181 (2012), Application(s): Assay, Abstract; Full Text
Aaptamine, an alkaloid from the sponge Aaptos suberitoides, functions as a proteasome inhibitor: S. Tsukamoto, et al.; Bioorg. Med. Chem. Lett. 20, 3341 (2010), Abstract;

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