Proteasome 19S Rpt2/S4 subunit, pAb



BML-PW8305-0025	25 µl	90.00 USD

BML-PW8305-0100	100 µl	312.00 USD

Replaces Prod. #: ALX-210-319

Product Specification

Formulation:	Liquid. In PBS containing 10mM sodium azide.

Immunogen:	Recombinant full length human proteasome 26S (19S regulator complex) (Rpt2/S4 subunit).

Source/Host:	From rabbit.

Specificity:	Recognizes human, mouse and rat proteasome 26S (19S regulator complex) (Rpt2/S4 subunit). Detects a band of ~55kDa by Western blot.

Application:	Immunoprecipitation Western Blot

Long Term Storage:	-20°C

Use/Stability:	Dilute with PBS, pH 7.2-7.4 containing 1% normal goat serum (if a goat anti-rabbit IgG linker antibody is to be used). Store diluted antibody at +4°C (do not freeze) and use within 1 month.

Handling:	After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.

Miscellaneous/General:	The proteasome is widely recognised as the central enzyme of non-lysosomal protein degradation. It is responsible for intracellular protein turnover and it is also critically involved in many regulatory processes and, in higher eukaryotes, in antigen processing. The 26S proteasome is the key enzyme of the ubiquitin/ATP-dependent pathway of protein degradation. The catalytic core of this unusually large (2000kDa, 450Å in length) complex is formed by the 20S proteasome, a barrel shaped structure shown by electron microscopy to comprise of four rings each containing seven subunits. Based on sequence similarity, all fourteen 20S proteasomal subunit sequences may be classified into two groups, α and β, each group having distinct structural and functional roles. The α-subunits comprise the outer rings and the β-subunits the inner rings of the 20S proteasome. Observations of the eukaryotic proteasome and analysis of subunit sequences indicate that each ring contains seven different subunits (α₇β₇β₇α₇) with a member of each sub-family represented in each particle. Each subunit is located in a unique position within the α- or β-rings.In addition to the 20S particle, the 26S complex contains over twenty additional proteins, ranging in molecular weight from 25 to 10kDa, located in a distinct complex called the ‘PA700 proteasome activator’ or the ‘19S complex’, and which determines substrate specificity and provides the multiple enzymatic functions necessary for proteolysis and viability. Systematic analysis of the sub-unit components have revealed at least six members to be ATPases belonging to a new family of ATP-binding proteins, together with a further fifteen sub-units that lack the capacity to bind ATP, isopeptidases and several other proteins thought to be responsible for the unfolding of a protein substrate prior to insertion into the proteolytic core of the 20S proteasome. Subunit roles and interactions are now being more fully defined.

Background / Technical Information:	Please click here for the comprehensive product datasheet.

Figure: Western blot analysis of human placental proteasome using Proteasome 19S Rpt2/S4 subunit, pAb (Prod. No. BML-PW8305).Method: SDS-PAGE was followed by blotting onto PVDF and probing with antibody BML-PW8305. Antibody dilution 1:1000 using ECL procedure.

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General Literature References

Mapping subunit contacts in the regulatory complex of the 26 S proteasome. S2 and S5b form a tetramer with ATPase subunits S4 and S7: C. Gorbea, et al.; J. Biol. Chem. 275, 875 (2000), Abstract; Full Text

Chromosomal localization and immunological analysis of a family of human 26S proteasomal ATPases: N. Tanahashi, et al.; BBRC 243, 229 (1998), Abstract;

Subunit arrangement in the human 20S proteasome: F. Kopp, et al.; PNAS 94, 2939 (1997), Abstract; Full Text

The 26S proteasome: subunits and functions: K. Tanaka & C. Tsurumi; Mol. Biol. Rep. 24, 3 (1997), Abstract;