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Proteasome 19S Rpt4/S10b subunit polyclonal antibody

 
BML-PW8220-0025 25 µl 105.00 USD
 
BML-PW8220-0100 100 µl 329.00 USD
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Replaces Prod. #: ALX-210-296

Product Specification

Alternative Name:Proteasome subunit p42, 26S protease regulatory subunit 10B, Proteasome 26S subunit ATPase 6
 
Host:Rabbit
 
Immunogen:Recombinant full length human p42.
 
UniProt ID:P62333
 
Species reactivity:Human
Yeast
 
Specificity:Recognizes the Rpt4/S10b subunit of the 19S regulator complex.
 
Applications:IHC, WB
 
Application Notes:Detects a band of ~44kDa by Western blot.
 
Formulation:Liquid. In PBS containing 10mM sodium azide.
 
Use/Stability:Dilute with PBS, pH 7.2-7.4 containing 1% normal goat serum (if a goat anti-rabbit IgG linker antibody is to be used). Store diluted antibody at +4°C (do not freeze) and use within 1 month.
 
Handling:Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Scientific Background:The proteasome is widely recognised as the central enzyme of non-lysosomal protein degradation. It is responsible for intracellular protein turnover and it is also critically involved in many regulatory processes and, in higher eukaryotes, in antigen processing. The 26S proteasome is the key enzyme of the ubiquitin/ATP-dependent pathway of protein degradation. The catalytic core of this unusually large (2000kDa, 450Å in length) complex is formed by the 20S proteasome, a barrel shaped structure shown by electron microscopy to comprise of four rings each containing seven subunits. Based on sequence similarity, all fourteen 20S proteasomal subunit sequences may be classified into two groups, α and β, each group having distinct structural and functional roles. The α-subunits comprise the outer rings and the β-subunits the inner rings of the 20S proteasome. Observations of the eukaryotic proteasome and analysis of subunit sequences indicate that each ring contains seven different subunits (α7β7β7α7) with a member of each sub-family represented in each particle. Each subunit is located in a unique position within the α- or β-rings. In addition to the 20S particle, the 26S complex contains over twenty additional proteins, ranging in molecular weight from 25 to 10kDa, located in a distinct complex called the ‘PA700 proteasome activator’ or the ‘19S complex’, and which determines substrate specificity and provides the multiple enzymatic functions necessary for proteolysis and viability. Systematic analysis of the sub-unit components have revealed at least six members to be ATPases belonging to a new family of ATP-binding proteins, together with a further fifteen sub-units that lack the capacity to bind ATP, isopeptidases and several other proteins thought to be responsible for the unfolding of a protein ubstrate prior to insertion into the proteolytic core of the 20S proteasomes.
 
bml-pw8220 wb
Figure: Western blot analysis of proteasome 19S Rpt4/S10b subunit from human brain extract using Proteasome 19S Rpt4/S10b subunit, pAb (Prod. No. BML-PW8220). Method: SDS-PAGE was followed by blotting onto nitrocellulose and probing with antibody BML-PW 8220. Antibody dilution 1:1,000 using ECL procedure (1 min exposure)
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bml-pw8220 wb

General Literature References

Subunit arrangement in the human 20S proteasome: F. Kopp, et al.; PNAS 94, 2939 (1997), Abstract; Full Text
The 26S proteasome: subunits and functions: K. Tanaka & C. Tsurumi; Mol. Biol. Rep. 24, 3 (1997), Abstract;

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