Online Purchasing Account You are logged on as Guest. LoginRegister a New AccountShopping cart (Empty)
United States 

Proteasome 20S α1, 2, 3, 5, 6 & 7 subunits monoclonal antibody (MCP231)

 
BML-PW8195-0025 25 µl 108.00 USD
 
BML-PW8195-0100 100 µl 345.00 USD
Do you need bulk/larger quantities?
 

Product Specification

Alternative Name:Proteasome subunit α type-1, -2, -3, -4, -5 & -6,
Macropain subunits C2, C3, C8, C9, ι & ζ
 
Clone:MCP231
 
Host:Mouse
 
Isotype:IgG1
 
Immunogen:Dinitrophenylated proteasomes.
 
UniProt ID:P25786 (human PSMA1), P25787 (human PSMA2), P25788 (human PSMA3), P25789 (human PSMA4), P28066 (human PSMA5), P60900 (human PSMA6)
 
Species reactivity:Human, Mouse, Rat
Potato, Rabbit, Yeast
 
Specificity:Recognizes the α1, 2, 3, 5, 6 & 7 subunits of the 20S proteasome.
 
Applications:IHC, WB
 
Purity Detail:Protein G affinity purified.
 
Formulation:Liquid. In PBS containing 0.01% sodium azide.
 
Use/Stability:Dilute in PBS pH 7.2-7.4 and 1% normal goat serum (if a goat anti-mouse IgG linker antibody is to be used)
 
Handling:Store unopened vial at -20°C until required for use. Aliquot after opening and store at 2-4°C. The use of high quaity \'antiserum-grade\' plastic or glass vials is recommended. Use within 1 month. Avoid freeze/thaw cycles.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Scientific Background:The proteasome is widely recognised as the central enzyme of non-lysosomal protein degradation. It is responsible for intracellular protein turnover and it is also critically involved in many regulatory processes and, in higher eukaryotes, in antigen processing. The 26S proteasome is the key enzyme of the ubiquitin/ATP-dependent pathway of protein degradation. The catalytic core of this unusually large (2000kDa, 450Å in length) complex is formed by the 20S proteasome, a barrel shaped structure shown by electron microscopy to comprise of four rings each containing seven subunits. Based on sequence similarity, all fourteen 20S proteasomal subunit sequences may be classified into two groups, α and β, each group having distinct structural and functional roles. The α-subunits comprise the outer rings and the β-subunits the inner rings of the 20S proteasome. Observations of the eukaryotic proteasome and analysis of subunit sequences indicate that each ring contains seven different subunits (α7β7β7α7) with a member of each sub-family represented in each particle. Each subunit is located in a unique position within the α- or β-rings. 120S Proteasomes degrade only unfolded proteins in an energy-independent manner, whereas 26S proteasomes degrade native and ubiquitinylated proteins in an ATP-dependent manner. The native protein substrates are recognised by subunits, some with ATP binding sites, of the outer 19S caps of the 26S proteasome. The hybridoma secreting the antibody to subunits HC2, HC3, HC8, HC9, Iota and Zeta was generated by fusion of spenocytes from Balb/c mice which had recieved repeated immunisation with dinitrophenylated proteasomes.
 
Technical Info/Product Notes:Various systems for the nomenclature of the proteasome subunits have been established. This may be a source of confusion as the system on UniProt differs from "standard" nomenclature as described in the literature. The UniProt ID and Gene Name will help to clearly identify the proteins.
 
bml-pw8195 b
Figure:Luminograph of human placental proteasome preparation after 2D PAGE followed by blotting onto nitrocellulose and probing with antibody Prod. No. BML-PW8195 (MCP231). Antibody dilution 1:1000 using ECL procedure (1 min exposure).
bml-pw8195 a
Figure:Ponceau S staining of human placental proteasome preparation after 2D PAGE followed by blotting onto nitrocellulose.
Please mouse over
bml-pw8195 b bml-pw8195 a

Product Literature References

Metformin treatment modulates the tumour-induced wasting effects in muscle protein metabolism minimising the cachexia in tumour-bearing rats: A.G. Oliveira, et al.; BMC Cancer 16, 418 (2016), Application(s): Immunoblotting, Abstract; Full Text
Importin-β facilitates nuclear import of human GW proteins and balances cytoplasmic gene silencing protein levels: D. Schraivogel, et al.; Nucleic Acids Res. 43, 7447 (2015), Application(s): Immunofluorescence, Abstract; Full Text
Sestrin 2 Regulates PDGF Receptor beta (PDGFRβ) Expression by Modulating Proteasomal and Nrf2 Transcription Factor Functions: A. Tomasovic, et al.; J. Biol. Chem. 290, 9738 (2015), Application(s): Western Blotting, Abstract; Full Text

General Literature References

Subunit arrangement in the human 20S proteasome: F. Kopp et al.; PNAS 94, 2939 (1997), Abstract;
The 26S proteasome: subunits and functions: K. Tanaka et al.; Mol. Biol. Rep. 24, 3 (1997), Abstract;
Human proteasomes analysed with monoclonal antibodies: K. B. Hendil et al.; Biochem. J. 305, 245 (1995), Abstract;

Related Products

Proteasome 20S (alpha subunits) antibody sampler pack 

Print as PDF
 
BML-PW8900-0001 8x10 µl 453.00 USD
Do you need bulk/larger quantities?
 

Product Toolbox

PRODUCT RESOURCES

Datasheet
MSDS
Certificate of Analysis

RELATED PRODUCTS

By target:
Proteasome 20S
By biological activity:
Proteasome 20S Monoclonal antibody
By catalog section:

PRODUCT SUPPORT

FAQs
Technical Service
Customer Service

Related Literature

Catalogs
Ubiquitin & UBL Signaling Catalog
Ubiquitin & UBL Signaling Catalog
Download as PDF

All new literature pieces

Recommend this page

 
For Research Use Only. Not for use in diagnostic procedures.
Keep in touch

©2017 Enzo Life Sciences, Inc.,