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Proteasome 19S Rpt3/S6b subunit polyclonal antibody

BML-PW8175-0025 25 µl 103.00 USD
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Product Specification

Alternative Name:Proteasome 26S subunit ATPase 4, Tat-binding protein 7, TBP7, 26S protease regulatory subunit 6B
Immunogen:Recombinant protein corresponding to the C-terminus of Manduca sexta proteasome 26S (19S regulator complex) (Rpt3/S6b subunit).
UniProt ID:P43686 (human)
Species reactivity:Human, Mouse, Rat
Specificity:Recognizes the Rpt3/S6b subunit of the 19S regulator complex.
Crossreactivity:Manduca proteins
Applications:IHC, WB
Application Notes:Single dimension SDS-PAGE of His6-tagged recombinant MS73 and His6-tagged recombinant human TBP7 followed by Western blotting at optimal dilution (1:1,000-1:5,000) gives single bands with a relative molecular weight of approximately 50kDa. Similarly, a human erythrocyte proteasome preparation gives a band at a relative molecular weight of ~48kDa (1:100). All signals are markedly reduced upon pre-adsorption of the antibody with recombinant protein.
Immunohistochemistry - This antibody has been used for the immunohistochemical analysis of Alzheimer's disease and dementia with Lewy bodies human brain sections. Recent work has also been successfully undertaken in mouse tissue.
Formulation:Liquid. In PBS containing 10mM sodium azide.
Use/Stability:Dilute with PBS, pH 7.2-7.4 containing 1% normal goat serum (if a goat anti-rabbit IgG linker antibody is to be used). Store diluted antibody at +4°C (do not freeze) and use within 1 month.
Handling:Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.
Shipping:Shipped on Blue Ice
Long Term Storage:-20°C
Scientific Background:The proteasome is widely recognised as the central enzyme of non-lysosomal protein degradation. It is responsible for intracellular protein turnover and it is also critically involved in many regulatory processes and, in higher eukaryotes, in antigen processing. The 26S proteasome is the key enzyme of the ubiquitin/ATP-dependent pathway of protein degradation. The catalytic core of this unusually large (2000kDa, 450Å in length) complex is formed by the 20S proteasome, a barrel shaped structure shown by electron microscopy to comprise of four rings each containing seven subunits. Based on sequence similarity, all fourteen 20S proteasomal subunit sequences may be classified into two groups, α and β, each group having distinct structural and functional roles.  The α-subunits comprise the outer rings and the β-subunits the inner rings of the 20S proteasome.  Observations of the eukaryotic proteasome and analysis of subunit sequences indicate that each ring contains seven different subunits (α7β7β7α7) with a member of each sub-family represented in each particle.  Each subunit is located in a unique position within the α- or β-rings. In addition to the 20S particle, the 26S complex contains over twenty additional proteins, ranging in molecular weight from 25 to 10kDa, located in a distinct complex called the ‘PA700 proteasome activator’ or the ‘19S complex’, and which determines substrate specificity and provides the multiple enzymatic functions necessary for proteolysis and viability.  Systematic analysis of the sub-unit components have revealed at least six members to be ATPases belonging to a new family of ATP-binding proteins, together with a further fifteen sub-units that lack the capacity to bind ATP, isopeptidases and several other proteins thought to be responsible for the unfolding of a protein substrate prior to insertion into the proteolytic core of the 20S proteasome.
bml-pw8175 wb
Figure: Western blot analysis of human erythrocyte proteasome preparation using Proteasome Rpt3/S6b subunit, pAb (Prod. No. BML-PW8175). Method: SDS-PAGE was followed by blotting onto PVDF and probing with antibody BML-PW8175. Antibody dilution 1:1000 using ECL procedure (1 min exposure).
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General Literature References

Subunit arrangement in the human 20S proteasome: F. Kopp, et al.; PNAS 94, 2939 (1997), Abstract; Full Text
The 26S proteasome: subunits and functions: K. Tanaka & C. Tsurumi; Mol. Biol. Rep. 24, 3 (1997), Abstract;
Pathological lesions of Alzheimer's disease and dementia with Lewy bodies brains exhibit immunoreactivity to an ATPase that is a regulatory subunit of the 26S proteasome: J. Fergusson, et al.; Neurosci. Lett. 219, 167 (1996), Abstract;
Developmental changes of the 26 S proteasome in abdominal intersegmental muscles of Manduca sexta during programmed cell death: S.P. Dawson, et al.; J. Biol. Chem. 270, 1850 (1995), Abstract; Full Text

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