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Mono- and polyubiquitinylated conjugates monoclonal antibody (FK2) (ATTO 488 conjugate)

 
BML-PW1335-0025 25 µl 140.00 USD
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  • Detects K29-, K48-, and K63-linked mono- and polyubiquitinylated proteins
  • Most cited multi-ubiquitin antibody with over 500 citations
  • Validated for WB, IP, IHC, and ELISA applications
The hybridoma secreting the MAb to Mono- and Polyubiquitinylated Conjugates (FK2) (Prod. No. BML-PW8810) was generated by fusion of splenocytes from Balb/c mice which had received repeated immunisation with a crude preparation of polyubiquitinylated-lysozyme. MAb to Mono- and Polyubiquitinylated Conjugates (FK2) has been extensively characterised by one-dimensional Western blotting and has been shown to recognise K29-, K48-, and K63-linked polyubiquitinylated and monoubiquitinylated proteins but not free ubiquitin.

Product Specification

Clone:FK2
 
Host:Mouse
 
Isotype:IgG1
 
Immunogen:Poly-ubiquitinylated lysozyme.
 
UniProt ID:P0CG47 (UBB), P0CG48 (UBC)
 
Species reactivity:Species independent
 
Specificity:Recognizes mono- and polyubiquitinylated conjugates.
 
Crossreactivity:Does not cross-react with free ubiquitin.
 
Applications:ICC
 
Recommended Dilutions/Conditions:Immunocytochemistry (1:250)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Purity Detail:Affinity purified.
 
Formulation:Liquid. In PBS containing 0.01% sodium azide.
 
Use/Stability:Stable for at least 1 year after receipt when stored at -20°C.
 
Handling:Avoid freeze/thaw cycles. Protect from light.
 
Shipping:Shipped on Blue Ice
 
Short Term Storage:+4°C
 
Long Term Storage:-20°C
 
BML-PW1335 ICC
Immmunocytochemistry Analysis: Detection of ubiquitinylated protein conjugates in HeLa cells by fluorescence microscopy using ATTO 488 labeled FK2 mAb (BML-PW1335), at 1:250 dilution. HeLa cells were treated with proteasome inhibitor MG-132 (5µM, BML-PI102) for 12 hours prior to analysis.
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BML-PW1335 ICC

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