Online Purchasing Account You are logged on as Guest. LoginRegister a New AccountShopping cart (Empty)
United States 

Ubiquitin-protein conjugates polyclonal antibody (fluorescein labeled)

 
BML-PW1235-0025 25 µl 300.00 USD
Do you need bulk/larger quantities?
 
For ubiquitin signaling pathway, selective autophagy, proteasomal degradation and protein aggregation studies using fluorescence microscopy.

Product Specification

Host:Rabbit
 
Immunogen:Ubiquitin.
 
UniProt ID:P0CG47 (UBB), P0CG48 (UBC), P62979 (RPS27A), P62987 (UBA52)
 
Species reactivity:Species independent
 
Specificity:Recognizes ubiquitin-protein conjugates and free ubiquitin.
 
Applications:ICC
 
Recommended Dilutions/Conditions:Immunocytochemistry (1:50-1:250)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Formulation:Liquid. In PBS containing 0.01% sodium azide as preservative.
 
Use/Stability: Stable for at least 1 year after receipt when stored at -20°C. Reagent is light sensitive; store in the dark. The use of high quality ‘antiserum-grade’ plastic or glass vials is recommended.
 
Handling:Protect from light. Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.
 
Shipping:Shipped on Blue Ice
 
Short Term Storage:+4°C
 
Long Term Storage:-20°C
 
Scientific Background:Ubiquitin modification of cellular proteins has been shown to be involved with a wide range of biochemical processes including proteasomal degradation, signal transduction, DNA repair, endocytosis and autophagy.
 
Technical Info/Product Notes:Ubiquitin polyclonal antibody BML-UG9510 was conjugated to fluorescein by a proprietary method. The resulting fluorescein-labelled ubiquitin polyclonal antibody (BML-PW1235) facilitates ready detection of ubiquitinylated protein conjugates in cells by fluorescence microscopy.
 
BML-PW1235 IF lr
Detection of ubiquitinylated protein conjugates in HeLa cells by fluorescence microscopy using fluorescein labeled ubiquitin pAb (BML-PW1235), at 1:250 dilution. HeLa cells were treated with proteasome inhibitor MG-132 (5µM, BML-PI102) for 12 hours prior to analysis.
Please mouse over
BML-PW1235 IF lr

General Literature References

A role for ubiquitin in selective autophagy: V. Kirkin, et al.; Mol.Cell. 34, 259 (2009), Abstract;
Proteasome-independent functions of ubiquitin in endocytosis and signaling: D. Mukhopadhyay & H. Riezman; Science 315, 201 (2007), Abstract;
TAK1 is a ubiquitin-dependent kinase of MKK and IKK: C. Wang et al. ; Nature 412, 346 (2001), Abstract;
The ubiquitin-proteasome pathway: on protein death and cell life: A. Ciechanover; EMBO J. 17, 7151 (1998), Abstract;

Related Literature

Brochures
Stem Cells
Stem Cells
Download as PDF

Catalogs
Ubiquitin & UBL Signaling Catalog
Ubiquitin & UBL Signaling Catalog
Download as PDF

Brochures
Essential Research Tools for Neurodegeneration & Neural Signaling
Essential Research Tools for Neurodegeneration & Neural Signaling
Download as PDF

All new literature pieces

Recommend this page

 
For Research Use Only. Not for use in diagnostic procedures.
Keep in touch

©2017 Enzo Life Sciences, Inc.,