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Ubiquitin-protein conjugates polyclonal antibody (fluorescein labeled)

BML-PW1235-0025 25 µl 300.00 USD
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For ubiquitin signaling pathway, selective autophagy, proteasomal degradation and protein aggregation studies using fluorescence microscopy.

Product Specification

UniProt ID:P0CG47 (UBB), P0CG48 (UBC), P62979 (RPS27A), P62987 (UBA52)
Species reactivity:Species independent
Specificity:Recognizes ubiquitin-protein conjugates and free ubiquitin.
Recommended Dilutions/Conditions:Immunocytochemistry (1:50-1:250)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Formulation:Liquid. In PBS containing 0.01% sodium azide as preservative.
Use/Stability: Stable for at least 1 year after receipt when stored at -20°C. Reagent is light sensitive; store in the dark. The use of high quality ‘antiserum-grade’ plastic or glass vials is recommended.
Handling:Protect from light. Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.
Shipping:Shipped on Blue Ice
Short Term Storage:+4°C
Long Term Storage:-20°C
Scientific Background:Ubiquitin modification of cellular proteins has been shown to be involved with a wide range of biochemical processes including proteasomal degradation, signal transduction, DNA repair, endocytosis and autophagy.
Technical Info/Product Notes:Ubiquitin polyclonal antibody BML-UG9510 was conjugated to fluorescein by a proprietary method. The resulting fluorescein-labelled ubiquitin polyclonal antibody (BML-PW1235) facilitates ready detection of ubiquitinylated protein conjugates in cells by fluorescence microscopy.
Ubiquitin-protein conjugates polyclonal antibody (fluorescein labeled) Immunofluorescence
Detection of ubiquitinylated protein conjugates in HeLa cells by fluorescence microscopy using fluorescein labeled ubiquitin pAb (BML-PW1235), at 1:250 dilution. HeLa cells were treated with proteasome inhibitor MG-132 (5µM, BML-PI102) for 12 hours prior to analysis.
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Ubiquitin-protein conjugates polyclonal antibody (fluorescein labeled) Immunofluorescence

General Literature References

A role for ubiquitin in selective autophagy: V. Kirkin, et al.; Mol.Cell. 34, 259 (2009), Abstract;
Proteasome-independent functions of ubiquitin in endocytosis and signaling: D. Mukhopadhyay & H. Riezman; Science 315, 201 (2007), Abstract;
TAK1 is a ubiquitin-dependent kinase of MKK and IKK: C. Wang et al. ; Nature 412, 346 (2001), Abstract;
The ubiquitin-proteasome pathway: on protein death and cell life: A. Ciechanover; EMBO J. 17, 7151 (1998), Abstract;

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