For ubiquitin signaling pathway, selective autophagy, proteasomal degradation and protein aggregation studies using fluorescence microscopy.
Product Specification
| Host: | Rabbit |
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| Immunogen: | Ubiquitin. |
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| UniProt ID: | P0CG47 (UBB), P0CG48 (UBC), P62979 (RPS27A), P62987 (UBA52) |
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| Species reactivity: | Species independent
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| Specificity: | Recognizes ubiquitin-protein conjugates and free ubiquitin. |
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| Applications: | ICC
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| Recommended Dilutions/Conditions: | Immunocytochemistry (1:50-1:250)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application. |
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| Formulation: | Liquid. In PBS containing 0.01% sodium azide as preservative. |
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| Use/Stability: |
Stable for at least 1 year after receipt when stored at -20°C. Reagent is light sensitive; store in the dark. The use of high quality ‘antiserum-grade’ plastic or glass vials is recommended. |
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| Handling: | Protect from light. Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C. |
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| Shipping: | Shipped on Blue Ice |
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| Short Term Storage: | +4°C |
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| Long Term Storage: | -20°C |
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| Scientific Background: | Ubiquitin modification of cellular proteins has been shown to be involved with a wide range of biochemical processes including proteasomal degradation, signal transduction, DNA repair, endocytosis and autophagy. |
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| Technical Info/Product Notes: | Ubiquitin polyclonal antibody BML-UG9510 was conjugated to fluorescein by a proprietary method. The resulting fluorescein-labelled ubiquitin polyclonal antibody (BML-PW1235) facilitates ready detection of ubiquitinylated protein conjugates in cells by fluorescence microscopy. |
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Detection of ubiquitinylated protein conjugates in HeLa cells by fluorescence microscopy using fluorescein labeled ubiquitin pAb (BML-PW1235), at 1:250 dilution. HeLa cells were treated with proteasome inhibitor MG-132 (5µM, BML-PI102) for 12 hours prior to analysis.
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General Literature References
A role for ubiquitin in selective autophagy: V. Kirkin, et al.; Mol.Cell.
34, 259 (2009),
Abstract;
Proteasome-independent functions of ubiquitin in endocytosis and signaling: D. Mukhopadhyay & H. Riezman; Science
315, 201 (2007),
Abstract;
TAK1 is a ubiquitin-dependent kinase of MKK and IKK: C. Wang et al. ; Nature
412, 346 (2001),
Abstract;
The ubiquitin-proteasome pathway: on protein death and cell life: A. Ciechanover; EMBO J.
17, 7151 (1998),
Abstract;
