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Nbr1 (human) polyclonal antibody

 
BML-PW1125-0025 25 µl 100.00 USD
 
BML-PW1125-0100 100 µl 356.00 USD
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For selective autophagy and protein aggregation studies.

Product Specification

Host:Rabbit
 
Immunogen:Synthetic peptide corresponding to amino acid residues 38-51 of human Nbr1 (UniProt link Q14596: NBR1_HUMAN).
 
UniProt ID:Q14596
 
Species reactivity:Human
 
Applications:WB
 
Recommended Dilutions/Conditions:Western blot (1:1,000)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Purity Detail:Partially purified by salt precipitation.
 
Formulation:Liquid. In PBS containing 0.01% sodium azide.
 
Use/Stability:Stable for at least 1 year after receipt when stored at -20°C.  The use of high quality ‘antiserum-grade’ plastic or glass vials is recommended.
 
Handling:Protect from light. Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.
 
Shipping:Shipped on Blue Ice
 
Short Term Storage:+4°C
 
Long Term Storage:-20°C
 
Scientific Background:Autophagy is a highly conserved bulk protein degradation pathway responsible for the turnover of long-lived proteins, disposal of damaged organelles, and clearance of aggregate-prone proteins. Autophagy is involved in various physiological or pathological processes, such as development, host defense response, cancer and neuronal degenerative diseases. It involves concerted action of more than 20 specific autophagy (ATG) proteins that mediate the formation of a double-membrane vesicle, the autophagosome, which engulfs the substrate and delivers it to the lysosome for degradation.

Nbr1 (neighbor of BRCA1 gene 1) has been identified, together with p62, as a selective autophagy receptor, capable of simultaneously binding both ubiquitin and autophagy-specific LC3/GABARAP proteins. Nbr1 is recruited to ubiquitin-positive protein aggregates and degraded by autophagy through its interaction with the LC3/GABARAP component of the autophagosomal membrane.

Such receptor proteins provide a molecular link between ubiquitinylation and autophagy, facilitating the selective targeting of various types of cargo, ranging from protein aggregates to membrane-bound organelles and microbes for autophagic degradation.
 
Technical Info/Product Notes:BML-PW1125 has been characterised for Nbr1 reactivity by Western blotting of purified recombinant Nbr1 protein and cell lysate samples (cells pre-treated with autophagy inhibitor bafilomycin [BML-CM110] prior to lysis). Note: Treatment of cells with autophagy inhibitiors (e.g. bafilomycin, BML-CM110) may be required in order to detect Nbr1 due to the rapid turnover in untreated cells degradation by cellular autophagy.
 
PW1125 WB
Western blot detection of Nrb1 in HeLa S100 lysate (25μg, Prod. No. BML-SW8750) using BML-PW1125 at 1:1000 dilution.
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PW1125 WB

General Literature References

A role for NBR1 in autophagosomal degradation of ubiquitinated substrates: V. Kirkin, et al.; Mol. Cell. 33, 505 (2009), Abstract;
A role for ubiquitin in selective autophagy: V. Kirkin, et al.; Mol.Cell. 34, 259 (2009), Abstract;
NBR1 and p62 as cargo receptors for selective autophagy of ubiquitinated targets: T. Lamark, et al.; Cell Cycle 8, 1986 (2009), Abstract;
Autophagy fights disease through cellular self-digestion: M. Mizushima, et al.; Nature 451, 1069 (2008), Abstract;
Autophagosome formation: core machinery and adaptations: Z. Xie, et al.; Nat. Cell. Biol. 9, 1102 (2007), Abstract;
Autophagy in the eukaryotic cell: F. Reggiori, et al.; Eukaryot. Cell. 1, 11 (2002), Abstract;

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