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S5a (human) polyclonal antibody

BML-PW1045-0025 25 µl 132.00 USD
BML-PW1045-0100 100 µl 426.00 USD
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Product Specification

Alternative Name:Rpn10, MCB1, PSMD4, 26S proteasome regulatory subunit S5A, Mbp1, Sun1, p54, Antisecretory factor 1 (ASF1)
Immunogen:Full-length recombinant GST-tagged human S5a (Accession number: P55036).
UniProt ID:P55036
Species reactivity:Human
Specificity:Recognizes the Rpn10/S5a subunit of the 19S regulator complex.
Recommended Dilutions/Conditions:Western Blot (1:1,000)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Purity Detail:Purified by immunodepletion of GST-reactive antibodies followed by Protein A purification.
Formulation:Liquid. In PBS containing 0.01% sodium azide.
Handling:Store unopened vial at -20°C until required for use. Store diluted antibody at 2-4°C and use within 1 month. Avoid freeze/thaw cycles.
Shipping:Shipped on Blue Ice
Short Term Storage:-20°C
Long Term Storage:-20°C
Scientific Background:S5a/Rpn10 is a major ubiquitin binding protein that binds preferentially to polyubiquitin chains. It is found as a non-ATPase subunit of the 19S regulatory complex of the 26S proteasome where it functions as a receptor for proteins destined for proteolytic degradation.In yeast, the homologue of S5a, Rpn10, is required for degradation of a subset of cellular proteins by the proteasome.  Unlike other proteasome subunits, S5a exists predominantly as a free protein in the cytosol (i.e. not incorporated into the proteasome), though its precise role in the cytosol has yet to be fully elucidated. S5a contains two approximately 15 amino acid ubiquitin interacting motifs (UIM) which are responsible for its binding to polyubiquitin chains.
Detection of S5a (Rpn10) 19S proteasome regulator subunit in purified 26S proteasome sample (50ng, BML-PW9310) by Western blotting using BML-PW1045 at 1:1000 dilution.
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General Literature References

Multiubiquitin chain receptors define a layer of substrate selectivity in the ubiquitin-proteasome system: R. Verma, et al.; Cell 118, 99 (2004), Abstract;
Characterization of two polyubiquitin binding sites in the 26 S protease subunit 5a: P. Young, et al.; J. Biol. Chem. 273, 5461 (1998), Abstract;
ATPase and ubiquitin-binding proteins of the yeast proteasome: D.M. Rubin, et al.; Mol. Biol. Rep. 24, 17 (1997), Abstract;
The multiubiquitin-chain-binding protein Mcb1 is a component of the 26S proteasome in Saccharomyces cerevisiae and plays a nonessential, substrate-specific role in protein turnover: S. van Nocker, et al.; Mol. Cell Biol. 16, 6020 (1996),
A 26 S protease subunit that binds ubiquitin conjugates: Q. Deveraux, et al.; J. Biol. Chem. 269, 7059 (1994), Abstract;

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