Proteasome 20S β5t subunit (human), mAb (b8-17)



BML-PW1020-0025	25 µl	90.00 USD

BML-PW1020-0100	100 µl	312.00 USD

Product Specification

Alternative Name:	Proteasome 20S β8 subunit

Concentration:	1mg/ml

Purity Detail:	Ion exchange purified.

Purity:	≥95% (SDS-PAGE)

Formulation:	Liquid. In PBS containing 0.01% sodium azide.

Clone:	b8-17

Isotype:	Mouse IgG₁

Immunogen:	Recombinant human 20S proteasome β5t subunit.

Specificity:	Recognizes human and mouse 20S proteasome β5t subunit.

Application:	Western Blot (1:500-1:1’000) Optimal conditions must be determined individually for each application.

Short Term Storage:	+4°C

Long Term Storage:	-20°C

Use/Stability:	Stable for at least 1 year after receipt when stored at -20°C.

Handling:	After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.

Miscellaneous/General:	When cells are stimulated with pro-inflammatory cytokines, most of their constitutively expressed proteasomes are replaced with immunoproteasomes, which increase the production of peptides for presentation on MHC class I molecules. In addition, cortical thymic epithelial cells selectively express a type of proteasome known as the thymoproteasome, in which the β5i immunoproteasome subunit has been replaced by the thymus specific proteasome subunit β5t. The thymoproteasome is required for the positive selection of thymocytes and shaping the T cell receptor repertoire of cytotoxic T lymphocytes. In addition mice deficient in β5t display defective development of CD8(+) T cells in the thymus suggesting a key role for β5t and the thymoproteasome in generating MHC class I-restricted CD8(+) T cells.

Background / Technical Information:	The hybridoma secreting the antibody clone b8-17 was generated by fusion of splenocytes from Balb/cmice which had received repeated immunisation with recombinant human β5t subunit protein (accession number: A5LHX3). Immunoglobulin was purified from tissue culture supernatant by ion exchange chromatography.

Western blot showing detection of 20S proteasome subunit β5t in C57/BL6 mouse thymus lysate by BML-PW1020 at 1:1000 dilution.

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General Literature References

Proteasomes in immune cells: more than peptide producers?: M. Groettrup, et al.; Nat. Rev. Immunol. 10, 73 (2010), Abstract;

Thymoproteasome shapes immunocompetent repertoire of CD8+ T cells: T. Nitta, et al.; Immunity 32, 29 (2010), Abstract;

Exclusive expression of proteasome subunit {beta}5t in the human thymic cortex : U. Tomaru, et al.; Blood 113, 5186 (2009), Abstract;

Regulation of CD8+ T cell development by thymus-specific proteasomes: S. Murata, et al.; Science 316, 1349 (2007), Abstract;

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