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Huwe1 (mouse) polyclonal antibody

 
BML-PW0950-0025 25 µl 100.00 USD
 
BML-PW0950-0100 100 µl 393.00 USD
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Product Specification

Alternative Name:HECT, UBA and WWE domain-containing protein 1, Upstream regulatory element-binding protein 1, Mcl-1 ubiquitin ligase E3, ARF-binding protein 1, Large structure of UREB1, Homologous to E6AP carboxyl terminus homologous protein 9, E3-histone
 
Host:Rabbit
 
Immunogen:Recombinant N-terminal fragment (aa 1367-1552) of mouse Huwe1 protein (accession number: Q7TMY8).
 
UniProt ID:Q7TMY8
 
Species reactivity:Human, Mouse, Rat
 
Applications:WB
 
Recommended Dilutions/Conditions:Western Blot (an initial dilution of 1:100 is recommended due to the dilute nature of the immunoaffinity purified antibody)
Note: Huwe1 is a large (485kDa), labile protein that is susceptible to fragmentation upon freeze thaw and upon heating. DO NOT heat lysate/protein samples when preparing for SDS-PAGE.
Western blotting conditions appropriate for the transfer of large proteins must be used to ensure good transfer of Huwe1 protein to immunoblotting membranes. For example use BSN transfer buffer 48mM TRIS, p9.2, 39mM glycine with 10% MeOH and 0.0375% SDS.
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Purity Detail:Antigenic protein fragment affinity purified.
 
Formulation:Liquid. In 100mM TRIS, pH 7.0, containing 100mM glycine and 0.02% sodium azide.
 
Use/Stability:Stable for at least 6 months after receipt when stored at 4°C.
 
Handling:Do not freeze.
 
Shipping:Blue Ice Not Frozen
 
Short Term Storage:+4°C
 
Long Term Storage:+4°C
 
Scientific Background:Ubiquitin E3 ligase that ubiquitinylates a range of target proteins involved in various cellular processes including MCL1 (apoptosis regulation), p53 (tumor suppression), core histones H1, H2A, H2B, H3 and H4 (chromatin modification), MYCN (neural differentiation regulation) and CDC6 (DNA damage response). Has been shown to be overexpressed in many human tumors and to play a role in spermatogenesis and neurogenesis.
 
bml-pw0950 wb-huwe1-pab
Detection of full length Huwe1 (~482kDa) and its degradation fragments in mouse testis lysate (50µg) by Western blotting using PW0950 at 1:100 dilution.
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bml-pw0950 wb-huwe1-pab

General Literature References

The N-Myc-DLL3 cascade is suppressed by the ubiquitin ligase Huwe1 to inhibit proliferation and promote neurogenesis in the developing brain: X. Zhao, et al.; Dev. Cell 17, 210 (2009), Abstract;
The HECT-domain ubiquitin ligase Huwe1 controls neural differentiation and proliferation by destabilizing the N-Myc oncoprotein: X. Zhao, et al.; Nat. Cell Biol. 10, 643 (2008), Abstract;
Cdc6 stability is regulated by the Huwe1 ubiquitin ligase after DNA damage: J.R. Hall, et al.; Mol. Biol. Cell 18, 3340 (2007), Abstract;
Regulated expression of the ubiquitin protein ligase, E3(Histone)/LASU1/Mule/ARF-BP1/HUWE1, during spermatogenesis: Z. Liu, et al.; Dev. Dyn. 236, 2889 (2007), Abstract;
Characterization of E3Histone, a novel testis ubiquitin protein ligase which ubiquitinates histones: Z. Liu, et al.; Mol. Cell Biol. 25, 2819 (2005), Abstract;
Mule/ARF-BP1, a BH3-only E3 ubiquitin ligase, catalyzes the polyubiquitination of Mcl-1 and regulates apoptosis: Q. Zhong, et al.; Cell 121, 1085 (2005), Abstract;
Over-expression of human UREB1 in colorectal cancer: HECT domain of human UREB1 inhibits the activity of tumor suppressor p53 protein: S.Y. Yoon, et al.; BBRC 326, 7 (2005), Abstract;
The ubiquitin ligase HectH9 regulates transcriptional activation by Myc and is essential for tumor cell proliferation: S. Adhikary, et al.; Cell 123, 409 (2005), Abstract;

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