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USP18 (human) polyclonal antibody

 
BML-PW0945-0025 25 µl 100.00 USD
 
BML-PW0945-0100 100 µl 330.00 USD
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Product Specification

Alternative Name:UBP43, ISG15 specific protease
 
Host:Rabbit
 
Immunogen:Synthetic peptide corresponding to aa 89-100 of human USP18.
 
UniProt ID:Q9UMW8
 
Species reactivity:Human
 
Applications:WB
 
Recommended Dilutions/Conditions:Western Blot (1:1,000)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Purity Detail:Purified by salt precipitation.
 
Formulation:Liquid. In PBS containing 0.01% sodium azide.
For further dilutions use PBS pH 7.4 and 1% normal goat serum (if a goat anti-rabbit IgG linker antibody is to be used).
 
Use/Stability:Stable for at least 1 year after receipt when stored as recommended.
 
Handling:Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Scientific Background:USP18 (UBP43) is a specific deconjugating enzyme for ISG15, a vertebrate ubiquitin like protein strongly upregulated by type I interferon following bacterial or viral infection. Whilst the molecular and regulatory consequences of ISGylation are not fully understood, the absence of USP18 has a profound effect on innate immunity, leading to increased resistance to certain viral infections. Notably, these effects do not appear to be contingent upon the proteolytic activity of USP18 suggesting additional roles for USP18 over and above that of an ISG15 specific protease. USP18 has been shown to significantly limit operation of the extrinsic apoptotic pathway triggered by type I interferon and drugs.
 
BML-PW0945 WB
Figure: Detection of purified recombinant USP18 (0.5µg) by Western blot using USP18 (human), pAb (Prod. No. BML-PW0945) (1:1’000).
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BML-PW0945 WB

General Literature References

Identification of USP18 as an important regulator of the susceptibility to IFN-alpha and drug-induced apoptosis: H. Potu, et al.; Cancer Res. 70, 655 (2010), Abstract;
UBP43 is a novel regulator of interferon signaling independent of its ISG15 isopeptidase activity: O.A. Malakhova, et al.; EMBO J. 25, 2358 (2006), Abstract;
ISG15: the immunological kin of ubiquitin: K.J. Ritchie & D.E. Zhang; Semin. Cell Dev. Biol. 15, 237 (2004), Abstract;
Role of ISG15 protease UBP43 (USP18) in innate immunity to viral infection: K.J. Ritchie, et al.; Nat. Med. 10, 1374 (2004), Abstract;

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