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Ubiquitin monoclonal antibody (P4D1)

BML-PW0930-0100 100 µg 164.00 USD
BML-PW0930-1000 1 mg 991.00 USD
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Product Specification

Immunogen:Denatured bovine ubiquitin.
UniProt ID:P0CG53 (UBB), P0CH28 (UBC), P62992 (RPS27A), P63048 (UBA52)
Species reactivity:Species independent
Specificity:Recognizes mono- and poly-ubiquitin protein conjugates, free polyubiquitin chains and free ubiquitin.
Applications:IHC, IP, WB
Recommended Dilutions/Conditions:Western Blot (1:1,000)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Application Notes:Clone P4D1 has been reported to be of utility in immunoprecipitation, immunofluorescence and immunohistochemistry (see Brady et al. (2005); Wang et al. (2008); Ito et al. (2003) respectively).
Purity Detail:Protein G affinity purified form cell culture media.
Formulation:Liquid. In PBS containing 0.01% sodium azide.
Use/Stability:Stable for at least 1 year after receipt when stored at -20°C.
Handling:Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.
Shipping:Shipped on Blue Ice
Short Term Storage:-20°C
Long Term Storage:-20°C
Scientific Background:Ubiquitin modification of cellular proteins has been shown to be involved with a wide range of biochemical processes including proteasomal degradation, signal transduction, DNA repair, endocytosis and autophagy.
Technical Info/Product Notes: The ubiquitin antibody BML-PW0930 (clone P4D1) was developed using a denatured glutaraldehyde cross-linked ubiquitin IgG complex as the immunogen. Monoclonal antibody was purified from tissue culture supernatant by ion exchange chromatography. The general ubiquitin reactivity of the monoclonal antibody has been demonstrated by Western blotting using synthetic ubiquitin chains, single isopeptide-linked polyubiquitin chains, and with endogenously ubiquitinylated species in cell lysates.

Product Literature References

Novel Regulatory Roles of Wnt1 in Infection-Associated Colorectal Cancer: J. Wang, et al.; Neoplasia 20, 499 (2018), Abstract; Full Text
FADD regulates NF-κB activation and promotes ubiquitination of cFLIPL to induce apoptosis: K. Ranjan, et al.; Sci. Rep. 6, 22787 (2016), Application(s): Western blot, Abstract; Full Text
NEDD4-mediated HSF1 degradation underlies α-synucleinopathy: E. Kim, et al.; Hum. Mol. Genet. 25, 211 (2016), Application(s): In vivo ubiquitination assay, Abstract; Full Text
UBXN2A regulates nicotinic receptor degradation by modulating the E3 ligase activity of CHIP: Y. Teng, et al.; Biochem. Pharmacol. 97, 518 (2015), Application(s): Immunoblotting, Abstract;

General Literature References

A role for ubiquitin in selective autophagy: V. Kirkin, et al.; Mol. Cell. 34, 259 (2009), Abstract;
Analysis of nondegradative protein ubiquitylation with a monoclonal antibody specific for lysine-63-linked polyubiquitin: H. Wang, et al.; PNAS 105, 20197 (2008), Abstract;
Proteasome-independent functions of ubiquitin in endocytosis and signaling: D. Mukhopadhyay & H. Riezman; Science 315, 201 (2007), Abstract;
Regulation of p53 and MDM2 activity by MTBP: M. Brady, et al.; Mol. Cell Biol. 25, 545 (2005), Abstract;
Dorfin localizes to Lewy bodies and ubiquitylates synphilin-1: T. Ito, et al.; J. Biol. Chem. 278, 29106 (2003), Abstract;
TAK1 is a ubiquitin-dependent kinase of MKK and IKK: C. Wang, et al.; Nature 412, 346 (2001), Abstract;
DOT4 links silencing and cell growth in Saccharomyces cerevisiae: A. Kahana & D.E. Gottschling; Mol. Cell Biol. 19, 6608 (1999), Abstract;
The ubiquitin-proteasome pathway: on protein death and cell life: A. Ciechanover; EMBO J. 17, 7151 (1998), Abstract;

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