Use1 (human), pAb



BML-PW0770-0025	25 µl	92.00 USD

BML-PW0770-0100	100 µl	325.00 USD

Product Specification

Purity Detail:	Crude serum.

Formulation:	Liquid. In PBS containing 0.01% sodium azide.

Immunogen:	Synthetic peptide corresponding to residues 231-242 of human use1 protein.

Source/Host:	From rabbit.

Specificity:	Recognizes human use1 protein.

Application:	Western Blot (1:1’000) Optimal conditions must be determined individually for each application.

Short Term Storage:	+4°C

Long Term Storage:	-20°C

Use/Stability:	Stable for at least 1 year after receipt when stored at -20°C. Store diluted antibody at 2-4°C and use within 1 month. Dilute with PBS pH 7.2-7.4 and 1% normal goat serum (if a goat-anti-rabbit IgG linker antibody is to be used).

Handling:	After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.

Miscellaneous/General:	UBA6 (ubiquitin-like modifier activating enzyme-6, also known as UbE1L2) is a novel ubiquitin activating enzyme that is also capable of activating the ubiquitin-like protein FAT10 (human leukocyte antigen F-associated transcript 10), which, similarly to ubiquitin, may serve as a signal for proteasomal degradation. Only one UBA6 specific E2 conjugating enzyme, known as Use1 (Uba6-specific E2 enzyme 1) or UbE2Z, which is capable of accepting activated FAT10, has been identified to date.

Background / Technical Information:	SiRNA-mediated down-regulation of use1 mRNA results in a strong reduction of FAT10 conjugate formation suggesting that USE1 is the major E2 enzyme in the FAT10 conjugation cascade. Interestingly USE1 has been identified as the first known substrate of FAT10 conjugation, as it is efficiently auto-FAT10ylated. Co-expression of FAT10 and the linker protein NUB1L lead to a proteolytic down-regulation of Use1 suggesting that auto-modification with FAT10 serves as a negative auto-regulation of FAT10 conjugation. BML-PW0770 has been characterized by Western blotting against endogenous and overexpressed Use1 in HEK293 cell lysates.UniProt ID Q9H832: Use1 (human)

General Literature References

USE1 is a bispecific conjugating enzyme for ubiquitin and FAT10, which FAT10ylates itself in cis.: A. Aichem, et al.; Nat. Comm. 1, 1 (2010),

Activating the ubiquitin family: UBA6 challenges the field.: M. Groettrup, et al.; Trends Biochem. Sci. 33, 230 (2008), Abstract;

E1-L2 activates both ubiquitin and FAT10.: Y. H. Chiu, et al.; Mol. Cell 27, 1014 (2007), Abstract;

The UBA domains of NUB1L are required for binding but not for accelerated degradation of the ubiquitin-like modifier FAT10.: G. Schmidtke, et al.; J. Biol. Chem. 281, 20045 (2006), Abstract;

FAT10, a ubiquitin-independent signal for proteasomal degradation.: M. S. Hipp, et al.; Mol. Cell. Biol. 25, 3483 (2005), Abstract;

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