FAT10 (human), mAb (4F1)



BML-PW0765-0025	25 µl	90.00 USD

BML-PW0765-0100	100 µl	312.00 USD

Product Specification

Concentration:	1mg/ml

Purity Detail:	Protein G-affinity purified.

Purity:	≥95% (SDS-PAGE).

Formulation:	Liquid. In PBS containing 0.01% sodium azide.

Clone:	4F1

Isotype:	Mouse IgG2a.

Immunogen:	Recombinant human FAT10.

Source/Host:	From mouse. Purified from hybridoma tissue culture supernatant.

Specificity:	Recognizes human FAT10.

Application:	Immunocytochemistry: recommended initial dilution at least (1:100). Immunoprecipitation: 1-2µg per 500µg of lysate protein. Western Blot: recommended initial dilution at least (1:1’000). Optimal conditions must be determined individually for each application.

Short Term Storage:	+4°C

Long Term Storage:	-20°C

Use/Stability:	Stable for at least 1 year after receipt when stored at -20°C. Store diluted antibody at 2-4°C and use within 1 month.

Handling:	After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.

Miscellaneous/General:	FAT10 is a ubiquitin-like protein (UBL), that is encoded in the major histocompatibility complex class I locus and is synergistically inducible with IFN-α and TNF-α. It is composed of two ubiquitin-like domains and possesses a free C-terminal diglycine motif that is required for FAT10 conjugate formation. Conjugation with FAT10 provides an alternative, cytokine inducible, ubiquitin-independent targeting mechanism for protein degradation by the proteasome, mediated by factors such as NUB1L. Although its function has not been fully elucidated, FAT10 has been shown to play important roles in various cellular processes including cell cycle control, chromosomal stability and apoptosis, and its up-regulation has been observed in various forms of cancer and HIV-associated renal disease. FAT10 expression is negatively regulated by the p53 tumour suppressor protein.

Background / Technical Information:	The hybridoma secreting the antibody 4F1 was generated by fusion of splenocytes from FAT10-/- mice which had received repeated immunisation with recombinant human GST-FAT10 (accession number: O15205). ELISA was used to exclude hybridomas producing antibodies with anti-GST activity and the 4F1 clone was stabilised after subsequent rounds of subcloning. The antibody was purified from cell culture supernatant using protein G agarose affinity chromatography. The antibody has been characterized by Western blotting against HA-FAT10 transfected and TNF-α/IFN-γ treated HEK293T cell lysates, by immunofluorescence utilizing HA-FAT10 transfected HEK293T cells, and by Immunoprecipitation. UniProt ID: O15205: FAT10 (human)

General Literature References

USE1 is a bispecific conjugating enzyme for ubiquitin and FAT10, which FAT10ylates itself in cis.: A. Aichem, et al.; Nat. Comm. 1, 1 (2010),

FAT10 plays a role in the regulation of chromosomal stability.: J. Ren, et al.; J. Biol. Chem. 281, 11413 (2006), Abstract;

FAT10, a gene up-regulated in various cancers, is cell-cycle regulated.: C.B. Lim, et al.; Cell Div. 1, 20 (2006), Abstract;

FAT10/diubiquitin-like protein-deficient mice exhibit minimal phenotypic differences.: A. Canaan, et al.; Mol. Cell. Biol. 26, 5180 (2006), Abstract;

p53 negatively regulates the expression of FAT10, a gene upregulated in various cancers.: D. W. Zhang, et al.; Oncogene 25, 16 (2006), Abstract;

Role of ubiquitin-like protein FAT10 in epithelial apoptosis in renal disease.: M. J. Ross, et al.; J. Am. Soc. Nephrol. 17, 996 (2006), Abstract;

The UBA domains of NUB1L are required for binding but not for accelerated degradation of the ubiquitin-like modifier FAT10.: G. Schmidtke, et al.; J. Biol. Chem. 281, 20045 (2006), Abstract;

FAT10, a ubiquitin-independent signal for proteasomal degradation.: M. S. Hipp, et al. ; Mol. Cell. Biol. 25, 3483 (2005), Abstract;

Expression of the FAT10 gene is highly upregulated in hepatocellular carcinoma and other gastrointestinal and gynecological cancers.: C. G. Lee, et al.; Oncogene 22, 2592 (2003), Abstract;

The ubiquitin-like protein FAT10 forms covalent conjugates and induces apoptosis.: S. Raasi, et al.; J. Biol. Chem. 276, 35334 (2001), Abstract;

A MHC-encoded ubiquitin-like protein (FAT10) binds noncovalently to the spindle assembly checkpoint protein MAD2.: Y. C. Liu, et al.; PNAS 96, 4313 (1999), Abstract;

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