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Mono- and polyubiquitinylated conjugates monoclonal antibody (FK2) (biotin conjugate)

 
BML-PW0755-0025 25 µl 108.00 USD
 
BML-PW0755-0100 100 µl 345.00 USD
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  • Detects K29-, K48-, and K63-linked mono- and polyubiquitinylated proteins
  • Most cited multi-ubiquitin antibody with over 500 citations
For detection of mono- and poly-ubiquitinylated protein conjugates Highly specific mouse monoclonal antibody, raised to a poly-ubiquitinylated protein, recognizes mono- and poly-ubiquitinylated proteins but not free ubiquitin. Biotin conjugate facilitates ready analysis using streptavidin-HRP detection system

Product Specification

Clone:FK2
 
Host:Mouse
 
Isotype:IgG1
 
Immunogen:Crude preparation of polyubiquitinylated-lysozyme.
 
UniProt ID:P0CG47 (UBB), P0CG48 (UBC)
 
Source:Purified from tissue culture supernatant. Biotin conjugated chemically in vitro.
 
Species reactivity:Species independent
 
Specificity:Recognizes polyubiquitinylated and monoubiquitinylated protein conjugates in a wide range of species.
 
Crossreactivity:Does not cross-react with free ubiquitin.
 
Applications:WB
 
Recommended Dilutions/Conditions:Western Blot (1:1,000)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Purity Detail:Ion exchange purified.
 
Formulation:Liquid. In PBS containing 0.01% sodium azide.
 
Use/Stability:Stable for at least 1 year after receipt when stored at -20°C.
 
Handling:Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.
 
Shipping:Shipped on Blue Ice
 
Short Term Storage:+4°C
 
Long Term Storage:-20°C
 
Technical Info/Product Notes:The hybridoma secreting the antibody FK2 was generated by fusion of splenocytes from Balb/c mice which had received repeated immunisation with a crude preparation of polyubiquitinylated-lysozyme. The antibody (clone FK2) has been extensively characterised by one-dimensional Western blotting and has been shown to recognise polyubiquitinylated (all linkage types) and monoubiquitinylated proteins but not free ubiquitin. FK2 antibody was conjugated to biotin by a proprietary method. The biotin-linked FK2 monoclonal antibody (FK2-biotin) facilitates ready detection of mono- and poly-ubiquitinylated protein conjugates and multi-ubiquitin chains by Western blotting using a Streptavidin-HRP detection system.
 
bml-pw0755 fk2-biotin
Figure: Western blot of K48-linked polyubiquitin chains (Ub2-7, Prod. No. BML-UW8860, 500ng) and K63-linked polyubiquitin chains (Ub2-7, Prod. No. BML-UW9570, 500ng) probed with MAb to Mono- and Polyubiquitinated Conjugates (FK2) (Biotin) (Prod. No. BML-PW0755) (1:1000 dilution), developed with streptavidin-HRP (Vectastain Elite ABC Kit, Vector Labs) and ECL Western blotting detection reagents.
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General Literature References

Dynamics of ubiquitin conjugation during heat-shock response revealed by using a monoclonal antibody specific to multi-ubiquitin chains: M. Fujimuro, et al.; Eur. J. Biochem. 249, 427 (1997), Abstract; Full Text
Serum concentrations of free ubiquitin and multiubiquitin chains: K. Takada, et al.; Clin. Chem. 43, 1188 (1997), Abstract; Full Text
Immunoassay for the quantification of intracellular multi-ubiquitin chains: K. Takada, et al.; Eur. J. Biochem. 233, 42 (1995), Abstract; Full Text
Production and characterization of monoclonal antibodies specific to multi-ubiquitin chains of polyubiquitinated proteins: M. Fujimuro, et al.; FEBS Lett. 349, 173 (1994), Abstract;

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