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AMSH (human) polyclonal antibody

 
BML-PW0655-0025 25 µl 100.00 USD
 
BML-PW0655-0100 100 µl 330.00 USD
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Product Specification

Alternative Name:STAMBP, STAM-binding protein
 
Host:Rabbit
 
Immunogen:Full-length human recombinant AMSH.
 
UniProt ID:O95630
 
Species reactivity:Human
 
Applications:WB
 
Recommended Dilutions/Conditions:Western Blot (1:1,000)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Purity Detail:Purified by salt precipitation and immunodepletion of GST reactive antibodies.
 
Formulation:Liquid. In PBS containing 0.01% sodium azide.
 
Use/Stability:Stable for at least 1 year after receipt when stored at -20°C. Dilute with PBS, pH 7.2-7.4, containing 1% normal goat serum (if a goat anti-rabbit IgG linker antibody is to be used). Store diluted antibody at +4°C, do not freeze and use within 1 month.
 
Handling:Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.
 
Shipping:Shipped on Blue Ice
 
Short Term Storage:+4°C
 
Long Term Storage:-20°C
 
Scientific Background:The JAMM domain containing zinc metalloprotease AMSH (Associated Molecule with the SH3-domain of STAM) acts as a deubiquitinylating enzyme during the sorting process to regulate receptor trafficking by specifically cleaving Lys63-linked polyubiquitin chains from internalized receptors. The amino-terminal half of AMSH proteins interacts with clathrin and ESCRT proteins on the endosome, whereas the C-terminal half is engaged in Lys63-linkage-specific deubiquitination. AMSH mediated cleavage of K63-linked ubiquitin chains is enhanced in the presence of its binding partner STAM.
 
bml-pw0655
Figure: Western blot showing detection of AMSH in HeLa S100 cell lysate (Prod. No. BML-SW8750, 2µg) with PAb to AMSH (Prod. No. BML-PW0655) at 1:1'000 dilution. Note: Band at ~35kDa is consistently observed with BML-PW0655 in different lysates and is thought to be a degradation product or splice variant of full length AMSH.
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General Literature References

Structural basis for specific cleavage of Lys 63-linked polyubiquitin chains: Y. Sato, et al.; Nature 455, 358 (2008), Abstract;
AMSH, an ESCRT-III associated enzyme, deubiquitinates cargo on MVB/late endosomes: M. Kyuuma, et al.; Cell Struct. Funct. 31, 159 (2007), Abstract;
Activation of the endosome-associated ubiquitin isopeptidase AMSH by STAM, a component of the multivesicular body-sorting machinery: J. McCullough, et al.; Curr. Biol. 16, 160 (2006), Abstract;
Endocytosis: the DUB version: M.J. Clague & S. Urbe; Trends Cell Biol. 16, 551 (2006), Abstract;
Interaction of AMSH with ESCRT-III and deubiquitination of endosomal cargo: M. Agromayor & J. Martin-Serrano; J. Biol. Chem. 281, 23083 (2006), Abstract;

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