AMSH (human), pAb



BML-PW0655-0025	25 µl	92.00 USD

BML-PW0655-0100	100 µl	325.00 USD

Product Specification

Alternative Name:	STAMBP, STAM-binding protein

Purity Detail:	Purified by salt precipitation and immunodepletion of GST reactive antibodies.

Formulation:	Liquid. In PBS containing 0.01% sodium azide.

Immunogen:	Full-length human recombinant AMSH.

Source/Host:	From rabbit.

Specificity:	Recognizes human AMSH.

Application:	Western Blot (1:1'000) Optimal conditions must be determined individually for each application.

Short Term Storage:	+4°C

Long Term Storage:	-20°C

Use/Stability:	Stable for at least 1 year after receipt when stored at -20°C. Dilute with PBS, pH 7.2-7.4, containing 1% normal goat serum (if a goat anti-rabbit IgG linker antibody is to be used). Store diluted antibody at +4°C, do not freeze and use within 1 month.

Handling:	After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.

Miscellaneous/General:	The JAMM domain containing zinc metalloprotease AMSH (Associated Molecule with the SH3-domain of STAM) acts as a deubiquitinylating enzyme during the sorting process to regulate receptor trafficking by specifically cleaving Lys⁶³-linked polyubiquitin chains from internalized receptors. The amino-terminal half of AMSH proteins interacts with clathrin and ESCRT proteins on the endosome, whereas the C-terminal half is engaged in Lys⁶³-linkage-specific deubiquitination. AMSH mediated cleavage of K^63-linked ubiquitin chains is enhanced in the presence of its binding partner STAM.

Figure: Western blot showing detection of AMSH in HeLa S100 cell lysate (Prod. No. BML-SW8750, 2µg) with PAb to AMSH (Prod. No. BML-PW0655) at 1:1\'000 dilution. Note: Band at ~35kDa is consistently observed with BML-PW0655 in different lysates and is thought to be a degradation product or splice variant of full length AMSH.

Please mouse over

General Literature References

Structural basis for specific cleavage of Lys 63-linked polyubiquitin chains: Y. Sato, et al.; Nature 455, 358 (2008), Abstract;

AMSH, an ESCRT-III associated enzyme, deubiquitinates cargo on MVB/late endosomes: M. Kyuuma, et al.; Cell Struct. Funct. 31, 159 (2007), Abstract;

Activation of the endosome-associated ubiquitin isopeptidase AMSH by STAM, a component of the multivesicular body-sorting machinery: J. McCullough, et al.; Curr. Biol. 16, 160 (2006), Abstract;

Endocytosis: the DUB version: M.J. Clague & S. Urbe; Trends Cell Biol. 16, 551 (2006), Abstract;

Interaction of AMSH with ESCRT-III and deubiquitination of endosomal cargo: M. Agromayor & J. Martin-Serrano; J. Biol. Chem. 281, 23083 (2006), Abstract;