USP21 (human), pAb



BML-PW0585-0025	25 µl	97.00 USD

BML-PW0585-0100	100 µl	341.00 USD

Product Specification

Alternative Name:	Ubiquitin thioesterase 21, Ubiquitin-specific-processing protease 21, Deubiquitinating enzyme 21, NEDD8-specific protease

Purity Detail:	Purified by salt precipitation.

Formulation:	Liquid. In PBS containing 0.01% sodium azide.

Immunogen:	Synthetic peptide corresponding to aa 60-74 of human USP21.

Source/Host:	From rabbit.

Specificity:	Recognizes human USP21.

Application:	Western Blot (1:1'000) Optimal conditions must be determined individually for each application.

Short Term Storage:	+4°C

Long Term Storage:	-20°C

Use/Stability:	Stable for at least 1 year after receipt when stored at -20°C. Dilute with PBS, pH 7.2-7.4, containing 1% normal goat serum (if a goat anti-rabbit IgG linker antibody is to be used). Store diluted antibody at +4°C, do not freeze and use within 1 month.

Handling:	After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.

Miscellaneous/General:	USP21 is a ubiquitin-specific protease that catalyzes the hydrolysis of ubH2A thereby acting to relieve ubH2A-specific repression. USP21 is also thought to be involved with hepatocyte regeneration. Furthermore, in addition to its deubiquitinylating activity, USP21 is capable of removing NEDD8 from NEDD8 conjugates but has no effect on SUMO conjugates.

Figure: Detection of USP21 in HeLa nuclear fraction (10µg) by Western blotting using PAb to USP21 (human) (Prod. No. BML-PW0585) at 1:1\'000 dilution. Note: The bands at ~40kDa are likely to be a truncated forms of the USP21 protein as both these and the full length USP21 band are removed by peptide blockade using the antigenic peptide.

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General Literature References

Deubiquitylation of histone H2A activates transcriptional initiation via trans-histone cross-talk with H3K4 di- and trimethylation: T. Nakagawa, et al.; Genes Dev. 22, 37 (2008), Abstract;

Chromatin modifications and their function: T. Kouzarides; Cell 128, 693 (2007), Abstract;

Identification of a novel isopeptidase with dual specificity for ubiquitin- and NEDD8-conjugated proteins: L. Gong, et al.; J. Biol. Chem. 275, 14212 (2000), Abstract;