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Blm10 (Saccharomyces cerevisiae) polyclonal antibody

 
BML-PW0570-0025 25 µl 121.00 USD
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Product Specification

Alternative Name:Blm3, PA200 homolog
 
Host:Rabbit
 
Immunogen:Recombinant N-terminal protein fragment (aa 1-157) of Saccharomyces cervisiae Blm10.
 
UniProt ID:P43583
 
Species reactivity:Saccharomyces cerevisiae
 
Applications:WB
 
Recommended Dilutions/Conditions:Western Blot (1:1,000)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Formulation:Liquid. Neat serum diluted in PBS containing 0.01% sodium azide.
 
Use/Stability:Stable for at least 1 year after receipt when stored at -20°C. Dilute with PBS, pH 7.2-7.4, containing 1% normal goat serum (if a goat anti-rabbit IgG linker antibody is to be used). Store diluted antibody at +4°C, do not freeze and use within 1 month. 
 
Handling:Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.
 
Shipping:Shipped on Blue Ice
 
Short Term Storage:+4°C
 
Long Term Storage:-20°C
 
Scientific Background:Blm10 (mammalian homolog PA200) is a large 246kDa regulatory protein that binds to the 20S proteasome core particle (CP). Blm10 recognizes pre-activated open gate CP which is assumed to exist in equilibrium with inactive closed gate CP. Blm10 distinguishes gate conformations, regulates CP activation, and has been shown to be involved in CP maturation.
 
bml-pw0570
Figure: Western blot detection of Blm10 containing complexes in GFP-labelled CP from wild type (1), blm10δ (2) and ump1δ (3) cells using PAb to Blm10 (Prod. No. BML-PW0570) at 1:1’000 dilution. Key features - A: Blm10-CP-RP, B: Blm102-CP, C: Blm10-CP
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Product Literature References

Dendritic cells maintain dermal adipose-derived stromal cells in skin fibrosis: J.J. Chia, et al.; J. Clin. Invest. 126, 4331 (2016), Application(s): Mouse injections treatments, Abstract; Full Text
Phosphorylation of the C-terminal tail of proteasome subunit α7 is required for binding of the proteasome quality control factor Ecm29: P. Wani, et al.; Sci. Rep. 6, 27873 (2016), Application(s): Immunoblotting, Abstract; Full Text
Maturation of the proteasome core particle induces an affinity switch that controls regulatory particle association: P.S. Wani, et al.; Nat. Commun. 6, 6384 (2015), Abstract;

General Literature References

Blm10 binds to pre-activated proteasome core particles with open gate conformation: A. Lehmann, et al.; EMBO Rep. 9, 1237 (2008), Abstract;
Mobilizing the proteolytic machine: cell biological roles of proteasome activators and inhibitors: M. Rechsteiner & C.P. Hill; Trends Cell Biol. 15, 27 (2005), Abstract;
Blm3 is part of nascent proteasomes and is involved in a late stage of nuclear proteasome assembly: M. Fehlker, et al.; EMBO Rep. 4, 959 (2003), Abstract; Full Text
PA200, a nuclear proteasome activator involved in DNA repair: V. Ustrell, et al.; EMBO J. 21, 3516 (2002), Abstract; Full Text

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