Blm10 (Saccharomyces cerevisiae), pAb

[Blm3, PA200 homolog]



BML-PW0570-0025	25 µl	89.00 USD

BML-PW0570-0100	100 µl	316.00 USD

Product Specification

Formulation:	Liquid. Neat serum diluted in PBS containing 0.01% sodium azide.

Immunogen:	Recombinant N-terminal protein fragment (aa 1-157) of Saccharomyces cervisiae Blm10.

Source/Host:	From rabbit.

Specificity:	Recognizes Saccharomyces cervisiae Blm10.

Application:	Western Blot (1:1’000) Optimal conditions must be determined individually for each application.

Shipping:	SHIPPED ON BLUE ICE

Short Term Storage:	+4°C

Long Term Storage:	-20°C

Use/Stability:	Stable for at least 1 year after receipt when stored at -20°C. Dilute with PBS, pH 7.2-7.4, containing 1% normal goat serum (if a goat anti-rabbit IgG linker antibody is to be used). Store diluted antibody at +4°C, do not freeze and use within 1 month.

Handling:	After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.

Miscellaneous/General:	Blm10 (mammalian homolog PA200) is a large 246kDa regulatory protein that binds to the 20S proteasome core particle (CP). Blm10 recognizes pre-activated open gate CP which is assumed to exist in equilibrium with inactive closed gate CP. Blm10 distinguishes gate conformations, regulates CP activation, and has been shown to be involved in CP maturation.

Figure: Western blot detection of Blm10 containing complexes in GFP-labelled CP from wild type (1), blm10δ (2) and ump1δ (3) cells using PAb to Blm10 (Prod. No. BML-PW0570) at 1:1’000 dilution. Key features - A: Blm10-CP-RP, B: Blm10₂-CP, C: Blm10-CP

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General Literature References

PA200, a nuclear proteasome activator involved in DNA repair V. Ustrell, et al. EMBO J. 21 3516 (2002) Abstract Full Text

Blm3 is part of nascent proteasomes and is involved in a late stage of nuclear proteasome assembly M. Fehlker, et al. EMBO Rep. 4 959 (2003) Abstract Full Text

Mobilizing the proteolytic machine: cell biological roles of proteasome activators and inhibitors M. Rechsteiner & C.P. Hill Trends Cell Biol. 15 27 (2005) Abstract

Blm10 binds to pre-activated proteasome core particles with open gate conformation A. Lehmann, et al. EMBO Rep. 9 1237 (2008) Abstract