Blm10 (Saccharomyces cerevisiae), pAb

BML-PW0570

Revised 01-Feb-10

Blm10 (Saccharomyces cerevisiae), pAb
SYNONYMS	Blm3 PA200 homolog
PRODUCT LINE	Proteasome & Related Complexes
PRODUCT CATEGORY	11S [PA28] Complex / Related Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
BML-PW0570-0025		25 µl	89.00 USD
BML-PW0570-0100		100 µl	316.00 USD

Product Specification

SOURCE/HOST:	From rabbit.
FORMULATION:	Liquid. Neat serum diluted in PBS containing 0.01% sodium azide.
IMMUNOGEN:	Recombinant N-terminal protein fragment (aa 1-157) of Saccharomyces cervisiae Blm10.
SPECIFICITY:	Recognizes Saccharomyces cervisiae Blm10.
APPLICATION:	Western Blot (1:1’000) Optimal conditions must be determined individually for each application.
SHIPPING:	SHIPPED ON BLUE ICE
SHORT TERM STORAGE:	+4°C
LONG TERM STORAGE:	-20°C
USE/STABILITY:	Stable for at least 1 year after receipt when stored at -20°C. Dilute with PBS, pH 7.2-7.4, containing 1% normal goat serum (if a goat anti-rabbit IgG linker antibody is to be used). Store diluted antibody at +4°C, do not freeze and use within 1 month.
HANDLING:	After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.

Product Images

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General Information

Blm10 (mammalian homolog PA200) is a large 246kDa regulatory protein that binds to the 20S proteasome core particle (CP). Blm10 recognizes pre-activated open gate CP which is assumed to exist in equilibrium with inactive closed gate CP. Blm10 distinguishes gate conformations, regulates CP activation, and has been shown to be involved in CP maturation.

General Literature References

PA200, a nuclear proteasome activator involved in DNA repair: V. Ustrell, et al.; EMBO J. 21, 3516 (2002) Abstract; Full Text
Blm3 is part of nascent proteasomes and is involved in a late stage of nuclear proteasome assembly: M. Fehlker, et al.; EMBO Rep. 4, 959 (2003) Abstract; Full Text
Mobilizing the proteolytic machine: cell biological roles of proteasome activators and inhibitors: M. Rechsteiner & C.P. Hill; Trends Cell Biol. 15, 27 (2005) Abstract
Blm10 binds to pre-activated proteasome core particles with open gate conformation: A. Lehmann, et al.; EMBO Rep. 9, 1237 (2008) Abstract

Further Categories Containing This Product:

Polyclonal Antibodies