Proteasome 19S Rpt2/S4 subunit (human), pAb



BML-PW0530-0025	25 µl	92.00 USD

BML-PW0530-0100	100 µl	325.00 USD

Product Specification

Alternative Name:	26S Proteasome subunit ATPase 1

Purity Detail:	Partially purified by salt precipitation.

Formulation:	Liquid. In PBS containing 0.01% sodium azide.

Immunogen:	Synthetic peptide corresponding to aa 368-383 of human proteasome 19S (Rpt2/S4 subunit).

Source/Host:	From rabbit.

Specificity:	Recognizes human proteasome 19S (Rpt2/S4 subunit).

Application:	Western Blot (1:1'000) Optimal conditions must be determined individually for each application.

Short Term Storage:	+4°C

Long Term Storage:	-20°C

Use/Stability:	Stable for at least 1 year after receipt when stored at -20°C. Dilute with PBS, pH 7.2-7.4, containing 1% normal goat serum (if a goat anti-rabbit IgG linker antibody is to be used). Store diluted antibody at +4°C, do not freeze and use within 1 month.

Handling:	After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.

Miscellaneous/General:	The 19S regulatory complex consists of a ‘base’ sub-complex, composed of a hetero-hexameric ring of AAA-ATPases that not only function as the gate-opener to the 20S proteasome but also, in conjunction with two other high-molecular-mass non-ATPase subunits (Rpn2/S1 and Rpn1/S2), as a platform for protein unfolding. The ‘lid’ sub-complex of the 19S complex, composed of non-ATPase subunits is involved in the binding and deubiquitinylation of proteasome targeted ubiquitin conjugated proteins and assists in the transport of substrates through the ‘base’ and into the 20S catalytic core.

Figure: Western blot showing detection of Rpt2/S4 19S regulatory subunit. Lane 1: Purified 26S proteasome (Prod. No. BML-PW9310, 1µg). Lane 2: HeLa S100 cytosolic fraction (Prod. No. BML-SW8750, 10µg). PAb to Proteasome 19S (Rpt2/S4 Subunit) (human) (Prod. No. BML-PW0530) at 1:1’000 dilution.

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General Literature References

Depletion of 26S proteasomes in mouse brain neurons causes neurodegeneration and Lewy-like inclusions resembling human pale bodies: L. Bedford, et al.; J. Neurosci. 28, 8189 (2008), Abstract;