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SENP6 (human) polyclonal antibody

BML-PW0370-0025 25 µl 100.00 USD
BML-PW0370-0100 100 µl 340.00 USD
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Product Specification

Alternative Name:Sentrin-specific protease 6, SUSP1, SUMO-1-specific protease 1
Immunogen:Recombinant human SENP6 (aa 635-1112) corresponding to the catalytic domain.
UniProt ID:Q9GZR1
Species reactivity:Human
Recommended Dilutions/Conditions:Western Blot (1:10,000; ensure that blocking is performed with 5% dried Milk/PBS/0.05% Tween, not BSA/PBST)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Purity Detail:Purified by salt precipitation and immunodepleted for species that cross-react with other SENP family members.
Formulation:Liquid. In PBS containing 0.01% sodium azide.
Use/Stability:Stable for at least 1 year after receipt when stored at -20°C. Store diluted antibody at +4°C, do not freeze and use within 1 month.
Handling:Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.
Shipping:Shipped on Blue Ice
Short Term Storage:+4°C
Long Term Storage:-20°C
Scientific Background:SENP6 exhibits a preference for SUMO-2 and SUMO-3 in deconjugation reactions and SUMO-2/SUMO-3 chains in particular, whereas it processes SUMO precursor proteins poorly. SENP6 depletion leads to SUMO-2 and SUMO-3 accumulation in PML nuclear bodies.
bml-pw0370 wb
Detection of SENP6 in HeLa nuclear fraction by Western blotting using BML-PW0370 at 1:10000 dilution. Note: The band at ~55kDa is a non-specific band consistently observed in both SENP6 expressing and SENP6 knockdown cell lysates.
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bml-pw0370 wb

General Literature References

A stress-dependent SUMO4 sumoylation of its substrate proteins: W. Wei, et al.; BBRC 375, 454 (2008), Abstract;
Structure of the human SENP7 catalytic domain and poly-SUMO deconjugation activities for SENP6 and SENP7: C.D. Lima & D. Reverter; J. Biol. Chem. 283, 32045 (2008), Abstract;
Concepts in sumoylation: a decade on: R. Geiss-Friedlander & F. Melchior; Nat. Rev. Mol. Cell Biol. 8, 947 (2007), Abstract;
Modification in reverse: the SUMO proteases: D. Mukhopadhyay & M. Dasso; Trends Biochem. Sci. 32, 286 (2007), Abstract;
SUMO-specific protease 1 is essential for stabilization of HIF1alpha during hypoxia: J. Cheng, et al.; Cell 131, 584 (2007), Abstract;
SUMO protease SENP1 induces isomerization of the scissile peptide bond: L. Shen, et al.; Nat. Struct. Mol. Biol. 13, 1069 (2006), Abstract;
SUMO-specific proteases and the cell cycle. An essential role for SENP5 in cell proliferation: A. Di Bacco & G. Gill; Cell Cycle 5, 2310 (2006), Abstract;
SUSP1 antagonizes formation of highly SUMO2/3-conjugated species: D. Mukhopadhyay, et al.; J. Cell Biol. 174, 939 (2006), Abstract;
SUMO, ubiquitin’s mysterious cousin: S. Muller, et al.; Nat. Rev. Mol. Cell Biol. 2, 202 (2001), Abstract;

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